Increased expression of intestinal P-selectin and pulmonary E-selectin during intravenous total parenteral nutrition
| Autor: | Henry G. Wilcox, Kazuhiko Fukatsu, Kenneth A. Kudsk, D. N. Granger, M. K. Hanna, Yong Wu, A. O. Gaber, Andrew H. Lundberg |
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| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_specialty P-selectin Intercellular Adhesion Molecule-1 Mice Random Allocation Reference Values Internal medicine E-selectin Intestine Small medicine Animals Intestine Large Infusions Intravenous Lung Probability Analysis of Variance biology business.industry Stomach Small intestine Surgery Diet P-Selectin medicine.anatomical_structure Endocrinology Parenteral nutrition Models Animal biology.protein Parenteral Nutrition Total business Pancreas |
| Zdroj: | Archives of surgery (Chicago, Ill. : 1960). 135(10) |
| ISSN: | 0004-0010 |
| Popis: | Hypothesis Intravenous total parenteral nutrition (TPN) induces intestinal polymorphonuclear neutrophil recruitment with increased intestinal intercellular adhesion molecule-1 expression. While intercellular adhesion molecule-1 causes firm adhesion of leukocytes to the endothelial cells, P- and E-selectin mediate leukocyte recruitment via rolling. Therefore, manipulation of nutrition may also affect P- and E-selectin expression in organs. Design Prospective randomized experimental trials. Setting Laboratory. Materials Male mice. Interventions Fifty-three mice were randomized to chow, intravenous TPN, or intragastric TPN. Main Outcome Measures After 5 days of diet, mice were administered iodine 125–labeled anti–P-selectin antibody (or iodine 125–labeled anti–E-selectin antibody) and iodine 131–labeled nonbinding antibody to quantify P-selectin (or E-selectin) expression in organs (lung, liver, kidney, small intestine, colon, stomach, pancreas, mesentery, heart, and skeletal muscle). Results P-selectin in small intestine, colon, stomach, and pancreas in the intravenous TPN group increased significantly as compared with the chow and the intragastric TPN groups. E-selectin expression was up-regulated after intravenous TPN in the lung but not in other sites. Conclusions In a time frame (5 days) when intercellular adhesion molecule-1 expression and neutrophil recruitment are increased, intestinal expression of P-selectin remains up-regulated. Early lung inflammatory changes are reflected by increases in E-selectin. This change may reflect early pulmonary dysfunction with intravenous TPN, but its significance requires further study. |
| Databáze: | OpenAIRE |
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