Taenia crassiceps: Serum and surface immunoglobulins in metacestode infections of mice
| Autor: | R.R. Blitz, Craig T. Morita, A.E. Siebert, A.H. Good |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Globulin
Secondary infection Immunology Immunoglobulins Receptors Antigen B-Cell Immunofluorescence Mice Antigen parasitic diseases medicine Animals Cytotoxic T cell Taeniasis Radial immunodiffusion Taenia crassiceps Mice Inbred BALB C Taenia biology medicine.diagnostic_test General Medicine biology.organism_classification Immunoglobulin A Infectious Diseases Immunoglobulin M Immunoglobulin G Larva biology.protein Female Parasitology Antibody |
| Zdroj: | Experimental Parasitology. 51:418-430 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/0014-4894(81)90129-6 |
| Popis: | Serum levels of IgM, IgA, IgG1, IgG2a, IgG2b, and IgG3 were measured weekly for 8 weeks by radial immunodiffusion in pooled sera from female BALB/c and BDF1 mice with primary and secondary Taenia crassiceps infections and age-matched normal control mice of each strain. Although increases in levels of all immunoglobulin classes occurred during primary and secondary infections in both strains of mice, the only consistent changes common to both strains of mice were higher levels of IgG1 and IgG3 in early weeks of secondary infections as compared to primary infections, and high levels of IgG1 late in primary infections. High levels of IgG3 occurred late in primary infections in BDF1 mice but not in BALB/c mice. It was not possible to correlate increased levels of any one immunoglobulin class either with cytotoxic activity of early immune serum or with the onset of the cellular encapsulation response in secondary infections. IgM, IgA, IgG1, IgG2a, IgG2b, and IgG3 could be demonstrated on the surface of washed fixed larvae from long-term infected donor mice by the indirect fluorescent antibody method. Living T. crassiceps larvae were capable of shedding fluorescent label within 1 hr at room temperature, but not at 4 C after staining with either rabbit anti-T. crassiceps serum or rabbit anti-mouse immunoglobulin serum and fluorescein-conjugated goat anti-rabbit globulin. |
| Databáze: | OpenAIRE |
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