A Manganese-Superoxide Dismutase From Thermus thermophilus HB27 Suppresses Inflammatory Responses and Alleviates Experimentally Induced Colitis
| Autor: | Bingxian Xie, Fan-Guo Meng, Shuai Chen, Wen Wei, Minjun Liu, Hong Yu Wang, Yang Sheng, Hai-Long Li, Jia-Wei Wu |
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| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Inflammation Pharmacology medicine.disease_cause Inflammatory bowel disease Superoxide dismutase Mice medicine Animals Immunology and Allergy Colitis Zebrafish chemistry.chemical_classification Enterocolitis Reactive oxygen species biology Superoxide Dismutase Chemistry Macrophages Thermus thermophilus Dextran Sulfate Gastroenterology biology.organism_classification medicine.disease Mice Inbred C57BL Oxidative Stress Neutrophil Infiltration Trinitrobenzenesulfonic Acid biology.protein medicine.symptom Reactive Oxygen Species Oxidative stress |
| Zdroj: | Inflammatory Bowel Diseases. 25:1644-1655 |
| ISSN: | 1536-4844 1078-0998 |
| DOI: | 10.1093/ibd/izz097 |
| Popis: | Background Superoxide dismutase (SOD) is an attractive therapeutic agent to ameliorate oxidative stress that is critical for the initiation and progression of inflammatory bowel disease (IBD). However, the short life of SOD limits its clinical application. In this study, we aim to examine the therapeutic effects of a hyperthermostable SOD from the Thermus thermophilus HB27 (TtSOD) for treatment of experimentally induced IBD. Methods A recombinant TtSOD was expressed and purified from Escherichia coli, and its therapeutic effects were examined in 2 experimental IBD animal models. Results In IBD induced by 2,4,6-trinitrobenzenesulfonic acid in zebrafish, TtSOD treatment decreased intestinal enlargement and attenuated neutrophil infiltration, resulting in alleviation of enterocolitis. In mice, SOD activity was substantially increased in the intestine after oral gavage of TtSOD, which ameliorated gut inflammation, preserved gut barrier function, and attenuated the severity of dextran sulfate sodium–induced colitis. Furthermore, TtSOD inhibited lipopolysaccharide-induced production of reactive oxygen species and inflammatory responses in mouse bone marrow–derived macrophages. Conclusions Our results demonstrate that TtSOD possesses therapeutic activities toward experimentally induced IBD, offering new clinical treatment options for patients with IBD. |
| Databáze: | OpenAIRE |
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