Mice derived from in vitro αMEM-cultured preimplantation embryos exhibit postprandial hyperglycemia and higher inflammatory gene expression in peripheral leukocytes
| Autor: | Teruhiko Wakayama, Mayu Kimura, Sachi Matsumoto, Nodoka Umihira, Kazuki Mochizuki, Shiori Ishiyama, Atsushi Takahashi, Takao Nakagawa, Satoshi Kishigami |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Blood Glucose Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Tissue Culture Techniques Mice 0302 clinical medicine Pregnancy Gene expression Leukocytes Organic Chemicals food and beverages Embryo General Medicine Postprandial Period Postprandial Prenatal Exposure Delayed Effects embryonic structures NADPH Oxidase 2 Tumor necrosis factor alpha Female medicine.symptom Biotechnology medicine.medical_specialty 030209 endocrinology & metabolism Inflammation Biology S100A8 03 medical and health sciences Internal medicine medicine Animals Calgranulin A Molecular Biology Gene Tumor Necrosis Factor-alpha Organic Chemistry Hordeum Oryza Atherosclerosis Embryo Transfer Embryo Mammalian In vitro Diet 030104 developmental biology Endocrinology Blastocyst Gene Expression Regulation Hyperglycemia |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 85(5) |
| ISSN: | 1347-6947 |
| Popis: | We examined whether peripheral leukocytes of mice derived from in vitro αMEM-cultured embryos and exhibiting type 2 diabetes had higher expression of inflammatory-related genes associated with the development of atherosclerosis. Also, we examined the impact of a barley diet on inflammatory gene expression. Adult mice were produced by embryo transfer, after culturing two-cell embryos for 48 h in either α minimal essential media (α-MEM) or potassium simplex optimized medium control media. Mice were fed either a barley or rice diet for 10 weeks. Postprandial blood glucose and mRNA levels of several inflammatory genes, including Tnfa and Nox2, in blood leukocytes were significantly higher in MEM mice fed a rice diet compared with control mice. Barley intake reduced expression of S100a8 and Nox2. In summary, MEM mice exhibited postprandial hyperglycemia and peripheral leukocytes with higher expression of genes related to the development of atherosclerosis, and barley intake reduced some gene expression. |
| Databáze: | OpenAIRE |
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