Discovery, Biocatalytic Exploration and Structural Analysis of a 4-Ethylphenol Oxidase from Gulosibacter chungangensis
| Autor: | Alejandro Gran-Scheuch, Mohammad Saifuddin, Milos Trajkovic, Alvigini Laura, Marco W. Fraaije, Andrea Mattevi, Yiming Guo |
|---|---|
| Přispěvatelé: | Biotechnology |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Oxidase test 4-Vinylphenol Molecular Structure Bioconversion Stereochemistry Organic Chemistry Penicillium Electron acceptor Alkenes Biochemistry Enzyme structure Hydroxylation Actinobacteria chemistry.chemical_compound Alcohol Oxidoreductases chemistry Phenols Biocatalysis Hydroxybenzoates Molecular Medicine Dehydrogenation Oxidoreductases Molecular Biology |
| Zdroj: | ChemBioChem. WILEY-V C H VERLAG GMBH ChemBioChem 2021, 22 'ChemBioChem ', vol: 22, pages: 3225-3233 (2021) |
| ISSN: | 1439-7633 1439-4227 |
| Popis: | The vanillyl-alcohol oxidase (VAO) family is a rich source of biocatalysts for the oxidative bioconversion of phenolic compounds. Through genome mining and sequence comparisons, we found that several family members lack a generally conserved catalytic aspartate. This finding led us to study a VAO-homolog featuring a glutamate residue in place of the common aspartate. This 4-ethylphenol oxidase from Gulosibacter chungangensis (Gc4EO) shares 42 % sequence identity with VAO from Penicillium simplicissimum, contains the same 8 alpha-N-3-histidyl-bound FAD and uses oxygen as electron acceptor. However, Gc4EO features a distinct substrate scope and product specificity as it is primarily effective in the dehydrogenation of para-substituted phenols with little generation of hydroxylated products. The three-dimensional structure shows that the characteristic glutamate side chain creates a closely packed environment that may limit water accessibility and thereby protect from hydroxylation. With its high thermal stability, well defined structural properties and high expression yields, Gc4EO may become a catalyst of choice for the specific dehydrogenation of phenolic compounds bearing small substituents. |
| Databáze: | OpenAIRE |
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