A rotating bottle culture method with continuous replacement of the gas phase
Autor: | D. L. Cockroft, D. A. T. New |
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Rok vydání: | 1979 |
Předmět: |
Pharmacology
business.product_category Chromatography Continuous flow chemistry.chemical_element Cell Biology Carbon Dioxide Hydrogen-Ion Concentration Oxygen Incubation period Gas phase Cellular and Molecular Neuroscience chemistry.chemical_compound chemistry Culture Techniques Carbon dioxide Bottle Animals Molecular Medicine Ectogenesis Constant (mathematics) business Molecular Biology Cells Cultured |
Zdroj: | Experientia. 35:138-140 |
ISSN: | 1420-9071 0014-4754 |
DOI: | 10.1007/bf01917926 |
Popis: | A 'rotator' culture method is described which provides a continuous flow of oxygenating gas to cultures in rotating bottles. The system maintains constant O2 and CO2 levels in the culture medium throughout the incubation period. It also provides a more stable pH than systems with sealed culture bottles. |
Databáze: | OpenAIRE |
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