Development of a Novel Experimental In Vitro Model of Isothiocyanate-induced Apoptosis in Human Malignant Melanoma Cells
| Autor: | Rodrigo Franco, Stavros Vasileiadis, S Perente, Ioannis Anestopoulos, Melina Mitsiogianni, Constantinos Simopoulos, Theodora Mantso, Aglaia Pappa, Aithne Atkinson, Mihalis I. Panayiotidis, Sotirios Botaitis, Aristeidis P. Sfakianos |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Phenethyl isothiocyanate B100 Apoptosis In Vitro Techniques 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Isothiocyanates Cell Line Tumor Humans 1112 Oncology and Carcinogenesis Oncology & Carcinogenesis Melanoma Caspase Dose-Response Relationship Drug biology Benzyl isothiocyanate General Medicine C400 HaCaT 030104 developmental biology Oncology chemistry Epidermoid carcinoma 030220 oncology & carcinogenesis Isothiocyanate Immunology Cancer research biology.protein Sulforaphane |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.11226 |
| Popis: | Background: Isothiocyanates are constituents of cruciferous vegetables which have been associated with reduced cancer risk partially through their ability to induce apoptosis in malignant cells including melanoma. \ud \ud Materials and Methods: We have utilized human malignant melanoma (A375), epidermoid carcinoma (A431) and immortalized keratinocyte (HaCaT) cells exposed to various isothiocyanates, under different experimental conditions. \ud \ud Results: An experimental in vitro model utilizing low isothiocyanate concentrations (0.1-5 μM for 48 h with all treatments being refreshed after 24h) was shown to be (i) most efficient in exerting an anti-cancer effect when compared to higher concentrations (5-100 μM for 24 or 48 h added as a single bolus) and (ii) specific to A375 cells while A431 and HaCaT cells remained unaffected. Such effect involved the activation of several caspases including (iii) initiator caspases 8, 9, 4 (indicating the involvement of intrinsic, extrinsic and endoplasmic reticulum-based pathways) and (iv) effector caspases 3, 7 and 6. \ud \ud Conclusion: Utilization of low isothiocyanate concentrations (under the conditions described herein) exerts an anti-cancer effect specific to human malignant melanoma cells thus providing a therapeutic basis for their utilization in management of the disease. |
| Databáze: | OpenAIRE |
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