DNA Origami Nanoantennas with over 5000-fold Fluorescence Enhancement and Single-Molecule Detection at 25 μM
| Autor: | Guillermo P. Acuna, Carolin Vietz, Philip Tinnefeld, Anastasiya Puchkova, Enrico Pibiri, Maria Sanz Paz, Bettina Wünsch |
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| Rok vydání: | 2015 |
| Předmět: |
Fluorophore
Materials science Metal Nanoparticles Bioengineering Nanotechnology Fluorescence chemistry.chemical_compound Light source DNA origami Molecule General Materials Science Lithography Plasmon Microscopy Confocal business.industry Mechanical Engineering DNA General Chemistry Condensed Matter Physics Nanostructures chemistry Colloidal gold Optoelectronics Gold business |
| Zdroj: | Nano Letters. 15:8354-8359 |
| ISSN: | 1530-6992 1530-6984 |
| DOI: | 10.1021/acs.nanolett.5b04045 |
| Popis: | Optical nanoantennas are known to focus freely propagating light and reversely to mediate the emission of a light source located at the nanoantenna hotspot. These effects were previously exploited for fluorescence enhancement and single-molecule detection at elevated concentrations. We present a new generation of self-assembled DNA origami based optical nanoantennas with improved robustness, reduced interparticle distance, and optimized quantum-yield improvement to achieve more than 5000-fold fluorescence enhancement and single-molecule detection at 25 μM background fluorophore concentration. Besides outperforming lithographic optical antennas, DNA origami nanoantennas are additionally capable of incorporating single emitters or biomolecular assays at the antenna hotspot. |
| Databáze: | OpenAIRE |
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