Inactivation of Acinetobacter baumannii Biofilms on Polystyrene, Stainless Steel, and Urinary Catheters by Octenidine Dihydrochloride
| Autor: | Deepti Prasad Karumathil, Kumar Venkitanarayanan, Amoolya Narayanan, Sangeetha Ananda Baskaran, Mary Anne Roshni Amalaradjou, Meera Surendran Nair |
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| Rok vydání: | 2016 |
| Předmět: |
Acinetobacter baumannii
0301 basic medicine Microbiology (medical) Urinary system Disinfectant 030106 microbiology Microbiology biofilm 03 medical and health sciences chemistry.chemical_compound Confocal imaging nosocomial infections stainless steel Original Research urinary catheter biology Biofilm biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification octenidine dihydrochloride Multiple drug resistance Catheter 030104 developmental biology chemistry Octenidine dihydrochloride |
| Zdroj: | Frontiers in Microbiology |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2016.00847 |
| Popis: | Acinetobacter baumannii is a major nosocomial pathogen causing human infections with significant mortality rates. In most cases, infections are acquired through exposure to A. baumannii biofilms that persist on contaminated hospital equipment and surfaces. Thus, it is imperative to develop effective measures for controlling A. baumannii biofilms in nosocomial settings. This study investigated the efficacy of octenidine dihydrochloride (OH), a new generation disinfectant for reducing A. baumannii biofilms on polystyrene, stainless steel and catheters. OH at 0.3% (5 mM), 0.6% (10 mM), and 0.9% (15 mM) was effective in significantly inactivating A. baumannii biofilms on all tested surfaces (P < 0.05). Furthermore, OH was equally effective in inactivating biofilms of multidrug resistant and drug susceptible A. baumannii isolates. In addition, confocal imaging revealed the predominance of dead cells in the OH-treated samples in comparison to the control. Further, scanning electron microscopy of biofilms formed on catheters revealed that OH treatment significantly reduced A. baumannii biofilm populations in corroboration with our antibiofilm assay. These data underscore the efficacy of OH in inactivating A. baumannii biofilms, thereby suggesting its potential use as a disinfectant or a catheter lock solution to control A. baumannii infections. |
| Databáze: | OpenAIRE |
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