Adenovirus-Mediated Transfer of a Recombinant α1-Antitrypsin Gene to the Lung Epithelium in Vivo
| Autor: | L D Stratford-Perricaudet, Koichi Yoneyama, Ronald G. Crystal, L Stier, Paavo K. Paakko, Wolfgang Siegfried, Melissa A. Rosenfeld, Jean-Pierre Lecocq, Sophie Jallat, Michel Perricaudet, Pascale Gilardi, Kunihiko Yoshimura, Masashi Fukayama, Andrea Pavirani |
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| Rok vydání: | 1991 |
| Předmět: |
Cystic Fibrosis
Transcription Genetic Genetic enhancement Genetic Vectors DNA Recombinant Gene Expression Alpha (ethology) Bronchi Biology Transfection Virus Replication medicine.disease_cause Epithelium Adenoviridae In vivo medicine Animals Humans RNA Messenger Sigmodontinae Promoter Regions Genetic Lung Emphysema Messenger RNA Multidisciplinary Genetic Therapy Molecular biology Trachea medicine.anatomical_structure alpha 1-Antitrypsin Immunology Respiratory epithelium Respiratory tract |
| Zdroj: | Science. 252:431-434 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | The respiratory epithelium is a potential site for somatic gene therapy for the common hereditary disorders alpha 1-antitrypsin (alpha 1AT) deficiency and cystic fibrosis. A replication-deficient adenoviral vector (Ad-alpha 1AT) containing an adenovirus major late promoter and a recombinant human alpha 1AT gene was used to infect epithelial cells of the cotton rat respiratory tract in vitro and in vivo. Freshly isolated tracheobronchial epithelial cells infected with Ad-alpha 1AT contained human alpha 1AT messenger RNA transcripts and synthesized and secreted human alpha 1AT. After in vivo intratracheal administration of Ad-alpha 1AT to these rats, human alpha 1AT messenger RNA was observed in the respiratory epithelium, human alpha 1AT was synthesized and secreted by lung tissue, and human alpha 1AT was detected in the epithelial lining fluid for at least 1 week. |
| Databáze: | OpenAIRE |
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