Cyclooxygenase-2 inhibitory and antioxidant compounds from the truffleElaphomyces granulatus
| Autor: | Shabana I. Khan, Rita Stanikunaite, James M. Trappe, Samir A. Ross |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Antioxidant
medicine.medical_treatment HL-60 Cells Biology Pharmacognosy Syringaldehyde Antioxidants Cell Line Inhibitory Concentration 50 Mice chemistry.chemical_compound Ascomycota Gallic Acid medicine Animals Humans Medicinal fungi Food science Pharmacology chemistry.chemical_classification Ethanol Cyclooxygenase 2 Inhibitors Molecular Structure Metabolism Syringic acid Enzyme Biochemistry chemistry Benzaldehydes |
| Zdroj: | Phytotherapy Research. 23:575-578 |
| ISSN: | 1099-1573 0951-418X |
| DOI: | 10.1002/ptr.2698 |
| Popis: | The ethanol extract of fruiting bodies of Elaphomyces granulatus, a truffle-like fungus, was evaluated for cyclooxygenase-2 (COX-2) enzyme inhibitory and antioxidant activities. Inhibition of COX-2 activity was evaluated in mouse macrophages (RAW 264.7). The extract of E. granulatus caused a 68% inhibition of COX-2 activity at 50 microg/mL. Bioassay-guided investigation led to the isolation and identification of two active compounds, syringaldehyde and syringic acid. Syringaldehyde moderately inhibited COX-2 activity with an IC(50) of 3.5 microg/mL, while syringic acid strongly inhibited COX-2 activity with an IC(50) of 0.4 microg/mL. The antioxidant activity of the extract and isolated compounds was evaluated in HL-60 cells by the DCFH-DA method. The extract of E. granulatus showed a potent antioxidant effect, with an IC(50) of 41 microg/mL. Of the pure compounds, syringic acid displayed a strong antioxidant activity, with an IC(50) of 0.7 microg/mL, while syringaldehyde showed no activity in the assay. |
| Databáze: | OpenAIRE |
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