Photoaffinity labelling of androgen receptors from human foreskin and cultured genital fibroblasts with tritiated methyltrienolone [3H]R1881
| Autor: | G. L. Warne, R. James, S. Gyorki, John W. Funder |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class Photochemistry Biology Genitalia Male Biochemistry Foreskin Endocrinology Affinity chromatography Internal medicine Labelling medicine Humans Estrenes Receptor Fibroblast Gel electrophoresis Affinity Labels Fibroblasts Metribolone Androgen Molecular biology Androgen receptor Molecular Weight medicine.anatomical_structure Receptors Androgen Electrophoresis Polyacrylamide Gel |
| Zdroj: | Journal of steroid biochemistry. 25(3) |
| ISSN: | 0022-4731 |
| Popis: | Androgen receptors were partially purified by affinity chromatography of cytosols prepared from either normal foreskins or normal cultured fibroblasts. Partially purified receptors were covalently labelled with [3H]R1881 (tritiated methyltrienolone) by ultraviolet photoactivation. Gel electrophoresis of cytosols from both sources showed two peaks of specific binding, Mr approximately 40 K and approximately 85 K, under denaturing conditions. Photoaffinity labelling of the human androgen receptor may thus provide a useful tool in further studies on disorders of androgenization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|