Baseline Gut Microbiota Composition Is Associated with Major Infections Early after Hematopoietic Cell Transplantation
Autor: | Hemant S. Murthy, Nosha Farhadfar, Debra Lynch Kelly, John Lybarger, Yu Wang, Christian Jobin, Gary P. Wang, Raad Z. Gharaibeh, Gaurav Trikha, Josee Gauthier, John R. Wingard, Zeina Al-Mansour, Rachel C. Newsome, Andrew Kozlov |
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Rok vydání: | 2020 |
Předmět: |
Enterocolitis
Transplantation biology business.industry medicine.drug_class Bacteroidetes Antibiotics Hematopoietic Stem Cell Transplantation Hematology Colonisation resistance Neutropenia biology.organism_classification medicine.disease Gastrointestinal Microbiome Feces RNA Ribosomal 16S Immunology Medicine Humans Bacteroides fragilis medicine.symptom business Dysbiosis Febrile neutropenia |
Zdroj: | Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 26(11) |
ISSN: | 1523-6536 |
Popis: | Infection is a major cause of morbidity and mortality after hematopoietic cell transplantation (HCT). Gut microbiota (GM) composition and metabolites provide colonization resistance against dominance of potential pathogens, and GM dysbiosis following HCT can be deleterious to immune reconstitution. Little is known about the composition, diversity, and evolution of GM communities in HCT patients and their association with subsequent febrile neutropenia (FN) and infection. Identification of markers before HCT that predict subsequent infection could be useful in developing individualized antimicrobial strategies. Fecal samples were collected prospectively from 33 HCT recipients at serial time points: baseline, post-conditioning regimen, neutropenia onset, FN onset (if present), and hematologic recovery. GM was assessed by 16S rRNA sequencing. FN and major infections (ie, bloodstream infection, typhlitis, invasive fungal infection, pneumonia, and Clostridium difficile enterocolitis) were identified. Significant shifts in GM composition and diversity were observed during HCT, with the largest alterations occurring after initiation of antibiotics. Loss of diversity persisted without a return to baseline at hematologic recovery. GM in patients with FN was enriched in Mogibacterium, Bacteroides fragilis, and Parabacteroides distasonis, whereas increased abundance of Prevotella, Ruminococcus, Dorea, Blautia, and Collinsella was observed in patients without fever. A baseline protective GM profile (BPGMP) was predictive of protection from major infection. The BPGMP was associated with subsequent major infections with 77% accuracy and an area under the curve of 79%, with sensitivity, specificity, and positive and negative predictive values of 0.71, 0.91, 0.77, and 0.87, respectively. Our data show that large shifts in GM composition occur early after HCT, and differences in baseline GM composition are associated with the development of subsequent major infections. |
Databáze: | OpenAIRE |
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