A method for the simulation of normal, carrier and affected controls for PCR-RFLP screening of a genetic disease in dairy cattle

Autor:	P. N. Mukhopadhyaya, H. H. Mehta, R. N. Rathod
Rok vydání:	2000
Předmět:	DNA Mutational Analysis Leukocyte-Adhesion Deficiency Syndrome Glycine Cattle Diseases Disease Biology medicine.disease_cause Polymerase Chain Reaction chemistry.chemical_compound medicine Animals Point Mutation Genetic Testing Molecular Biology Dairy cattle DNA Primers Leukocyte adhesion deficiency Electrophoresis Agar Gel Genetics Aspartic Acid Mutation Genetic disorder Diagnostic test Cell Biology Reference Standards medicine.disease chemistry CD18 Antigens Cattle Restriction fragment length polymorphism Polymorphism Restriction Fragment Length DNA
Zdroj:	Molecular and Cellular Probes. 14:381-384
ISSN:	0890-8508
DOI:	10.1006/mcpr.2000.0329
Popis:	A technique is described that may be used to create in vitro mutations in PCR templates to generate affected and carrier controls for diagnostic testing when DNA from such individuals is not easily obtained. The method is demonstrated for a PCR-RFLP diagnostic test of the genetic disorder BLAD (Bovine Leukocyte Adhesion Deficiency).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::994b0a2349e21911f882dfceac85463b https://doi.org/10.1006/mcpr.2000.0329 Zobrazit plný text záznamu Full Text from ScienceDirect