Mast cells mediate neutrophil recruitment during atherosclerotic plaque progression

Autor: Daniël van der Velden, H. Maxime Lagraauw, Anouk Wezel, Johan Kuiper, Ilze Bot, Paul H.A. Quax, Saskia C.A. de Jager
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Male
Apolipoprotein E
Receptors
CXCR4
Chemokine
Neutrophils
Chemokine CXCL1
Population
Aortic Diseases
Atherosclerotic plaque development and progression
Research Support
CXCR4
Receptors
Interleukin-8B
Lesion
Influx
Peritoneal cavity
Apolipoproteins E
Paracrine Communication
Journal Article
Animals
Medicine
CXC chemokine receptors
Non-U.S. Gov't
education
Aorta
Cells
Cultured
Mice
Knockout
education.field_of_study
biology
business.industry
Research Support
Non-U.S. Gov't
Immunoglobulin E
Atherosclerosis
Mast cell
Chemokine CXCL12
Plaque
Atherosclerotic
Mice
Inbred C57BL
Disease Models
Animal
medicine.anatomical_structure
Neutrophil Infiltration
Immunology
Disease Progression
biology.protein
Mast cells
Recruitment
medicine.symptom
Chemokines
Cardiology and Cardiovascular Medicine
business
Signal Transduction
Zdroj: Atherosclerosis, 241(2), 289-296
Atherosclerosis, 241(2), 289. Elsevier
ISSN: 0021-9150
Popis: AIMS\nActivated mast cells have been identified in the intima and perivascular tissue of human atherosclerotic plaques. As mast cells have been described to release a number of chemokines that mediate leukocyte fluxes, we propose that activated mast cells may play a pivotal role in leukocyte recruitment during atherosclerotic plaque progression.\nMETHODS AND RESULTS\nSystemic IgE-mediated mast cell activation in apoE(-/-)μMT mice resulted in an increase in atherosclerotic lesion size as compared to control mice, and interestingly, the number of neutrophils was highly increased in these lesions. In addition, peritoneal mast cell activation led to a massive neutrophil influx into the peritoneal cavity in C57Bl6 mice, whereas neutrophil numbers in mast cell deficient Kit(W(-sh)/W(-sh)) mice were not affected. Within the newly recruited neutrophil population, increased levels of CXCR2(+) and CXCR4(+) neutrophils were observed after mast cell activation. Indeed, mast cells were seen to contain and release CXCL1 and CXCL12, the ligands for CXCR2 and CXCR4. Intriguingly, peritoneal mast cell activation in combination with anti-CXCR2 receptor antagonist resulted in decreased neutrophil recruitment, thus establishing a prominent role for the CXCL1/CXCR2 axis in mast cell-mediated neutrophil recruitment.\nCONCLUSIONS\nOur data suggest that chemokines, and in particular CXCL1, released from activated mast cells induce neutrophil recruitment to the site of inflammation, thereby aggravating the ongoing inflammatory response and thus affecting plaque progression and destabilization.
Databáze: OpenAIRE
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