Sugar sensing by enterocytes combines polarity, membrane bound detectors and sugar metabolism
| Autor: | Maude Le Gall, Vanessa Tobin, Edith Brot-Laroche, Emilie Stolarczyk, Armelle Leturque, Véronique Dalet |
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| Přispěvatelé: | Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ALFEDIAM Merck Lipha, Institut Benjamin Delessert, AIP ATC Nutrition, Grant Number: ASEO22129DSA., Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Le Gall, Maude |
| Rok vydání: | 2007 |
| Předmět: |
Sucrose
MESH : RNA Messenger [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] MESH : Promoter Regions Genetic MESH : Green Fluorescent Proteins MESH: Protein Structure Tertiary MESH: Enterocytes Mice 0302 clinical medicine Gene expression sugar metabolism MESH : Glucose Transporter Type 5 MESH: Animals MESH : Glucose Transporter Type 2 MESH : Sucrase MESH: Oligo-1 6-Glucosidase 0303 health sciences MESH: Sweetening Agents MESH : Enterocytes Jejunum MESH : Protein Structure Tertiary endocrine system MESH : Cloning Molecular Monosaccharide Transport Proteins MESH : Sodium-Glucose Transporter 1 Oligo-1 6-Glucosidase Transfection 03 medical and health sciences MESH: Green Fluorescent Proteins Sodium-Glucose Transporter 1 MESH: Promoter Regions Genetic Humans MESH: Cloning Molecular RNA Messenger [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Humans MESH : Glucose [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology MESH : Humans MESH: Sucrose MESH : Caco-2 Cells Fructose Protein Structure Tertiary Mice Inbred C57BL Enterocytes Glucose chemistry MESH: Jejunum Sweetening Agents GLUT2 MESH: Glucose Transporter Type 2 MESH: Glucose Transporter Type 5 GLUT5 MESH: Fructose 030217 neurology & neurosurgery Physiology [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition Clinical Biochemistry enterocyte MESH: Mice Knockout MESH : Fructose chemistry.chemical_compound glucose signalling MESH: Hexoses MESH : Jejunum Cloning Molecular Receptor Promoter Regions Genetic MESH : Oligo-1 6-Glucosidase Glucose Transporter Type 2 Mice Knockout biology [ SDV.MHEP.PHY ] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Glucose Transporter Type 5 Cell Polarity MESH: Glucose Biochemistry MESH: Monosaccharide Transport Proteins MESH: Sodium-Glucose Transporter 1 MESH: Caco-2 Cells MESH : Transfection MESH: Cell Polarity MESH : Cell Polarity Sucrase Green Fluorescent Proteins MESH : Mice Inbred C57BL [SDV.BC]Life Sciences [q-bio]/Cellular Biology MESH: Mice Inbred C57BL MESH : Mice [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology sweet taste receptor Sugar [SDV.BC] Life Sciences [q-bio]/Cellular Biology MESH: Mice 030304 developmental biology MESH : Monosaccharide Transport Proteins MESH: RNA Messenger Hexoses MESH: Transfection Cell Biology Metabolism Gustducin MESH : Sucrose [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition MESH : Sweetening Agents MESH: Sucrase biology.protein MESH : Mice Knockout MESH : Animals Caco-2 Cells MESH : Hexoses [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Journal of Cellular Physiology Journal of Cellular Physiology, Wiley, 2007, 213 (3), pp.834-43. ⟨10.1002/jcp.21245⟩ Journal of Cellular Physiology, 2007, 213 (3), pp.834-43. ⟨10.1002/jcp.21245⟩ Journal of Cellular Physiology, Wiley, 2007, 213 (3), pp.834-43. 〈10.1002/jcp.21245〉 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.21245⟩ |
| Popis: | Sugar consumption and subsequent sugar metabolism are known to regulate the expression of genes involved in intestinal sugar absorption and delivery. Here we investigate the hypothesis that sugar-sensing detectors in membranes facing the intestinal lumen or the bloodstream can also modulate intestinal sugar absorption. We used wild-type and GLUT2-null mice, to show that dietary sugars stimulate the expression of sucrase-isomaltase (SI) and L-pyruvate kinase (L-PK) by GLUT2-dependent mechanisms, whereas the expression of GLUT5 and SGLT1, did not rely on the presence of GLUT2. By providing sugar metabolites, sugar transporters, including GLUT2, fuelled a sensing pathway. In Caco2/TC7 enterocytes, we could disconnect the sensing triggered by detector from that produced by metabolism, and found that GLUT2 generated a metabolism-independent pathway to stimulate the expression of SI and L-PK. In cultured enterocytes, both apical and basolateral fructose could increase the expression of GLUT5, conversely, basolateral sugar administration could stimulate the expression of GLUT2. Finally, we located the sweet-taste receptors T1R3 and T1R2 in plasma membranes, and we measured their cognate Galpha Gustducin mRNA levels. Furthermore, we showed that a T1R3 inhibitor altered the fructose-induced expression of SGLT1, GLUT5, and L-PK. Intestinal gene expression is thus controlled by a combination of at least three sugar-signaling pathways triggered by sugar metabolites and membrane sugar receptors that, according to membrane location, determine sugar-sensing polarity. This provides a rationale for how intestine adapts sugar delivery to blood and dietary sugar provision. J. Cell. Physiol. 213:834–843. © 2007 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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