Influence of Fucoidan Extracts from Different Fucus Species on Adult Stem Cells and Molecular Mediators in In Vitro Models for Bone Formation and Vascularization
| Autor: | Andreas Seekamp, Sabine Fuchs, Susanne Alban, Julia Ohmes, Yuejun Xiao, Sandesh Neupane, Fanlu Wang, Signe Helle Ptak, Junyu Xiong, Ramona Römer, Xavier Fretté |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Angiogenesis
mesenchymal stem cells (MSC) Pharmaceutical Science Fucus vesiculosus Neovascularization Physiologic Angiogenesis Inhibitors 02 engineering and technology outgrowth endothelial cells (OEC) Article 03 medical and health sciences chemistry.chemical_compound angiogenesis fucoidan Osteogenesis Polysaccharides bone vascularization Drug Discovery Humans Angiogenic Proteins Bone regeneration Cytotoxicity Pharmacology Toxicology and Pharmaceutics (miscellaneous) lcsh:QH301-705.5 Cells Cultured 030304 developmental biology Cell Proliferation 0303 health sciences biology Chemistry Fucoidan Endothelial Cells Mesenchymal Stem Cells 021001 nanoscience & nanotechnology biology.organism_classification In vitro Coculture Techniques Vascular endothelial growth factor Molecular Weight Biochemistry lcsh:Biology (General) Fucus Alkaline phosphatase Inflammation Mediators 0210 nano-technology Energy Metabolism Signal Transduction |
| Zdroj: | Marine Drugs Marine Drugs, Vol 19, Iss 194, p 194 (2021) Volume 19 Issue 4 Wang, F, Xiao, Y, Neupane, S, Ptak, S H, Römer, R, Xiong, J, Ohmes, J, Seekamp, A, Fretté, X, Alban, S & Fuchs, S 2021, ' Influence of Fucoidan Extracts from Different Fucus Species on Adult Stem Cells and Molecular Mediators in In Vitro Models for Bone Formation and Vascularization ', Marine Drugs, vol. 19, no. 4, 194 . https://doi.org/10.3390/md19040194 |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md19040194 |
| Popis: | Fucoidans, sulfated polysaccharides extracted from brown algae, are marine products with the potential to modulate bone formation and vascularization processes. The bioactivity and safety of fucoidans are highly associated with their chemical structure, which may vary with algae species and extraction method. Thus, in depth evaluation of fucoidan extracts in terms of endotoxin content, cytotoxicity, and their detailed molecular biological impact on the individual cell types in bone is needed. In this study, we characterized fucoidan extracts from three different Fucus species including Fucus vesiculosus (Fv), Fucus serratus (Fs), and Fucus distichus subsp. evanescens (Fe) for their chemical features, endotoxin content, cytotoxicity, and bioactive effects on human outgrowth endothelial cells (OEC) and human mesenchymal stem cells (MSC) as in vitro models for bone function and vascularization. Extracts contained mainly high molecular weight (HMW) fucoidans and were free of endotoxins that may cause inflammation or influence vascularization. OEC tolerated fucoidan concentrations up to 200 µg/mL, and no indication of cytotoxicity was observed. The inflammatory response, however, investigated by real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) and endothelial barrier assessed by impedance measurement differed for the individual extracts. MSC in comparison with endothelial cells were more sensitive to fucoidans and showed partly reduced metabolic activity and proliferation at higher doses of fucoidans. Further results for MSC indicated impaired osteogenic functions in alkaline phosphatase and calcification assays. All tested extracts consistently lowered important molecular mediators involved in angiogenesis, such a VEGF (vascular endothelial growth factor), ANG-1 (angiopoietin 1), and ANG-2 (angiopoietin 2), as indicated by RT-PCR and ELISA. This was associated with antiangiogenic effects at the functional level using selected extracts in co-culture models to mimic bone vascularization processes during bone regeneration or osteosarcoma. |
| Databáze: | OpenAIRE |
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