Genotoxic Effects of Aluminum Chloride and Their Relationship with N-Nitroso-N-Methylurea (NMU)-Induced Breast Cancer in Sprague Dawley Rats
| Autor: | Agustín Gómez-Álvarez, Armando Burgos-Hernández, Karen Lillian Rodríguez-Martínez, Alejandro Monserrat García-Alegría, Iván Anduro-Corona, José Guillermo López-Cervantes, Humberto González-Ríos, Eduardo Ruiz-Bustos, Rafael Canett-Romero, Humberto Astiazaran-Garcia |
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| Rok vydání: | 2020 |
| Předmět: |
inorganic chemicals
medicine.medical_specialty Adenoma Health Toxicology and Mutagenesis H&E stain lcsh:Chemical technology Toxicology medicine.disease_cause Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Breast cancer Internal medicine medicine lcsh:TP1-1185 Serous gland genotoxicity aluminum chloride rats 030304 developmental biology 0303 health sciences Chemical Health and Safety Cell growth Chemistry N-Nitroso-N-methylurea medicine.disease Endocrinology 030220 oncology & carcinogenesis Micronucleus Genotoxicity |
| Zdroj: | Toxics; Volume 8; Issue 2; Pages: 31 Toxics, Vol 8, Iss 31, p 31 (2020) Toxics |
| ISSN: | 2305-6304 |
| DOI: | 10.3390/toxics8020031 |
| Popis: | Recently, soluble forms of aluminum for human use or consumption have been determined to be potentially toxic due to their association with hepatic, neurological, hematological, neoplastic, and bone conditions. This study aims to assess the genotoxic effect of aluminum chloride on genomic instability associated with the onset of N-nitroso-N-methylurea (NMU)-induced breast cancer in Sprague Dawley rats. The dietary behavior of the rats was assessed, and the concentration of aluminum in the mammary glands was determined using atomic absorption spectroscopy. Genomic instability was determined in the histological sections of mammary glands stained with hematoxylin and eosin. Moreover, micronucleus in peripheral blood and comet assays were performed. The results of dietary behavior evaluation indicated no significant differences between the experimental treatments. However, aluminum concentration in breast tissues was high in the +2000Al/−NMU treatment. This experimental treatment caused moderate intraductal cell proliferation, lymph node hyperplasia, and serous gland adenoma. Furthermore, micronucleus and comet test results revealed that +2000Al/−NMU led to a genotoxic effect after a 10-day exposure and the damage was more evident after a 15-day exposure. Therefore, in conclusion, genomic instability is present and the experimental conditions assessed are not associated with breast cancer. |
| Databáze: | OpenAIRE |
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