Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects
| Autor: | Cristian Alvarez Sedó, F. Noblia, D. Lorenzi, Florencia Nodar, H. Uriondo, Estefanía Ventimiglia Lagar, M. Bilinski, Valeria Longobucco |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Pregnancy Rate DNA damage Embryonic Development Apoptosis DNA fragmentation Fertilization in Vitro Biology Andrology 03 medical and health sciences 0302 clinical medicine Human fertilization blastulation rate Pregnancy medicine Humans Blastocyst Embryo Implantation Sperm Injections Intracytoplasmic 030219 obstetrics & reproductive medicine TUNEL assay Oocyte Donation blastocyst Pregnancy Outcome Embryo Middle Aged Sperm Spermatozoa 030104 developmental biology medicine.anatomical_structure Female Original Article |
| Zdroj: | JBRA Assisted Reproduction |
| ISSN: | 1518-0557 |
| Popis: | OBJECTIVE The aim of this study was to investigate the effect of sperm DNA fragmentation on fertilization rate, embryo development (blastulation rate), and pregnancy outcomes for ICSI cycles performed in a cohort of couples using donor eggs and to assess the remaining embryos that were not transferred or frozen for apoptotic markers. METHODS Eighty-two women (egg recipients) were included in the study (2016) were included in the study. The recipients' mean age was 41.8±5.1 y/o (36-49), while the egg donors' mean age was 30.8±2.1 y/o (27-33). Even though donor egg cycles with frozen sperm samples are performed regularly in our center, 35 cycles were done using fresh sperm samples. The mean age of the males involved in the procedure was 40.1±5.2 y/o. Fertilization, blastulation, and pregnancy rates were assessed. The patients were divided into two groups, TUNEL |
| Databáze: | OpenAIRE |
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