Comparison of DNA typing success in compromised blood and touch samples based on sampling swab composition
| Autor: | Carolyn A Lewis, Memunatu Koroma, Ciara Rhodes, Jordan O. Cox, Brittany C Hudson, Chastyn Smith, Tracey Dawson Cruz, Sarah J. Seashols-Williams |
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| Rok vydání: | 2021 |
| Předmět: |
Touch DNA
Sample (material) Biology 01 natural sciences Polymerase Chain Reaction Pathology and Forensic Medicine law.invention Specimen Handling 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Genetics Humans Sampling (medicine) 030216 legal & forensic medicine Typing Chromatography 010401 analytical chemistry DNA Degradation Necrotic Electrophoresis Capillary DNA DNA extraction DNA Fingerprinting 0104 chemical sciences chemistry Touch Cotton swab Sample collection Blood Chemical Analysis Microsatellite Repeats |
| Zdroj: | Journal of forensic sciencesREFERENCES. 66(4) |
| ISSN: | 1556-4029 |
| Popis: | Sample collection at the crime scene can introduce variations in DNA recovery based upon the substrate from which a sample is collected, the material of the collection device used, or the storage conditions after collection. There are many factors during this process that can degrade the sample during drying and storage, and before DNA extraction can be performed. The purpose of this study was to evaluate and compare the performance of standard cotton swab collection with the Bode BioSafe® swab, which includes both a desiccant at the swab head and proprietary compounds to prevent degradation of the sample during sample collection and preservation. Blood and touch DNA samples were collected from porous and nonporous substrates and stored at elevated temperatures to simulate accelerated time. DNA quantification and STR profile data were used to assess the performance of the swabs. BioSafe® swab collection resulted in similar DNA yields from blood samples and significantly higher DNA yields from touch samples when compared to collection with cotton swabs. BioSafe® swabs also resulted in higher DNA integrity during long-term storage, increased STR profile success and improved retention of low-level contributor alleles. |
| Databáze: | OpenAIRE |
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