Viral infection and Toll-like receptor agonists induce a differential expression of type I and λ interferons in human plasmacytoid and monocyte-derived dendritic cells
| Autor: | Marina Cella, Roberto Lande, Danièle Monneron, Maria Elena Remoli, Martina Severa, Elena Giacomini, Eliana M. Coccia, Ilkka Julkunen, Gilles Uzé |
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| Rok vydání: | 2004 |
| Předmět: |
Interferon Regulatory Factor-7
medicine.medical_treatment Immunology Gene Expression Receptors Cell Surface Biology Monocytes Cell Line Interferon Gene expression medicine Humans Immunology and Allergy RNA Messenger Receptor Cells Cultured Toll-like receptor Membrane Glycoproteins Toll-Like Receptors hemic and immune systems Dendritic Cells Dendritic cell Toll-Like Receptor 3 Cell biology DNA-Binding Proteins Toll-Like Receptor 4 Cytokine Influenza A virus Cell culture Toll-Like Receptor 9 Interferon Regulatory Factors Interferon Type I Interferons Transcription Factors medicine.drug Interferon regulatory factors |
| Zdroj: | European Journal of Immunology. 34:796-805 |
| ISSN: | 0014-2980 |
| Popis: | In humans, the type I interferon (IFN) family consists of 13 IFN-alpha subtypes, IFN-beta and IFN-omicron the newly discovered IFN-like family consists of IFN-lambda1, -lambda2 and -lambda3. We have investigated the expression of type I and lambda IFN genes following virus infections or Toll-like receptor (TLR) triggering in monocyte-derived DC (MDDC) and plasmacytoid DC (pDC). We found that all IFN-alpha, -beta, -omicron and -lambda subtypes are expressed in influenza-virus-infected MDDC or pDC. Conversely, differential type I IFN gene transcription was induced in MDDC and pDC stimulated by specific TLR agonists. TLR-9 stimulation by CpG DNA induced the expression of all IFN-alpha, -beta, -omicron and -lambda subtypes in pDC, whereas TLR-4 stimulation by LPS, or TLR-3 stimulation by poly I:C, induced only IFN-beta and IFN-lambda gene expression in MDDC. The expression pattern of IFN regulatory factor (IRF)-5 and IRF-7 in MDDC and pDC was also determined. IRF-5 was constitutively expressed in the two DC subsets whereas IRF-7 was constitutive in pDC but its expression was induced along MDDC maturation. Overall, our data indicate that the coordinated expression of IFN-lambda with IFN-beta would be of crucial importance for the maturation of DC. |
| Databáze: | OpenAIRE |
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