Expression of signal transductions proteins during the differentiation of primary human erythroblasts
| Autor: | Alessandro Matteucci, Viviana di Giacomo, Silvano Capitani, Elena Alfani, Lucio Cocco, Angela Di Baldassarre, Anna Rita Migliaccio, Giovanni Migliaccio, Emilia Stellacci, Angela Battistini |
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| Přispěvatelé: | Viviana Di Giacomo, Alessandro Matteucci, Emilia Stellacci, Angela Battistini, Angela Di Baldassarre, Silvano Capitani, Elena Alfani, Anna Rita Migliaccio, Lucio Cocco, Giovanni Migliaccio |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Erythroblasts
Physiology Cellular differentiation Clinical Biochemistry bcl-X Protein Biology Bioinformatics Isozyme DNA-binding protein Phosphatidylinositol 3-Kinases Antigens CD hemic and lymphatic diseases medicine Humans Erythropoiesis Cell Shape Cells Cultured Cell Differentiation Cell Biology Phosphoproteins Cell biology DNA-Binding Proteins Isoenzymes Basophilic STAT1 Transcription Factor IRF1 Proto-Oncogene Proteins c-bcl-2 Erythropoietin Type C Phospholipases Trans-Activators Signal transduction Biomarkers Interferon Regulatory Factor-1 Signal Transduction medicine.drug |
| Zdroj: | Journal of cellular physiology 202 (2005): 831–838. info:cnr-pdr/source/autori:V. DI GIACOMO, A. MATTEUCCI, E. STELLACCI, A. BATTISTINI, A. DI BALDASSARRE, S. CAPITANI, E. ALFANI, A.R. MIGLIACCIO, L. COCCO, G. MIGLIACCIO,/titolo:Expression of signal transduction proteins during the differentiation of primary human erythroblasts,/doi:/rivista:Journal of cellular physiology (Print)/anno:2005/pagina_da:831/pagina_a:838/intervallo_pagine:831–838/volume:202 |
| Popis: | The high number (>10(8-10)) of primary human pro-erythroblasts (CD36high/CD235alow) obtainable in HEMA culture (Migliaccio et al., 2002) is exploited here to analyse the expression of proteins implicated in erythropoietin (EPO)-signalling (STATs, PI-3K, and PLCs) during the process of erythroid maturation. Human pro-erythroblasts progressed in 4 days of culture with EPO into basophilic- (CD36high/CD235amedium, 24 h), polychromatic-(CD36high/CD235ahigh, 48 h), and, finally, orthochromatic-(CD36low/CD235ahigh, 72-96 h) erythroblasts. During this maturation, STAT-1 was expressed up to the orthochromatic stage, expression of STAT-5, as well as of its target proteins BclxL and IRF1, remained constant up to 48 h (polychromatic-erythroblasts) but decreased by 96 h (orthochromatic-erythroblasts), while that of STAT-3 decreased constantly from 24 h on and became undetectable by 96 h. Expression of PI-3K rapidly decreased with differentiation since only 50% of original protein levels were detected by 48 h. On the other hand, among the members of PLC families investigated, PLC beta4 was not expressed, PLC beta2, delta1, and gamma2 were expressed at constant levels throughout the maturation process, while expression of PLC beta3 and of PLC gamma1 decreased, as PI-3K, by 24 h and that of PLC beta1 was induced by 6 h and became undetectable by 24 h. In conclusion, these data depict the dynamic signalling scenario associated with the maturation of erythroid cells and provide the first indication that members of PLC families (PLC beta1, beta3, and gamma1) might be involved in the control of erythroid differentiation in humans. |
| Databáze: | OpenAIRE |
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