ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation
Autor: | David C. Yeomans, Christopher J. Benson, Kathleen A. Sluka, Kazimierz Babinski, Steven P. Wilson, Jayasheel O. Eshcol, Katherine M. Audette, Margaret P. Price, Rajan Radhakrishnan |
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Rok vydání: | 2007 |
Předmět: |
Pain Threshold
medicine.medical_specialty Patch-Clamp Techniques Enzyme-Linked Immunosorbent Assay Inflammation Sodium Channels Article Membrane Potentials Mice Ganglia Spinal Physical Stimulation Internal medicine Threshold of pain medicine Animals Simplexvirus RNA Messenger Patch clamp Muscle Skeletal Cells Cultured Acid-sensing ion channel Skin Mice Knockout Neurons Behavior Animal Reverse Transcriptase Polymerase Chain Reaction business.industry Chronic pain medicine.disease Immunohistochemistry Acid Sensing Ion Channels Mice Inbred C57BL Anesthesiology and Pain Medicine medicine.anatomical_structure Endocrinology Neurology Hyperalgesia Immunology Nociceptor Neurology (clinical) Neuron medicine.symptom business |
Zdroj: | Pain. 129:102-112 |
ISSN: | 0304-3959 |
Popis: | Peripheral initiators of muscle pain are virtually unknown, but likely key to development of chronic pain after muscle insult. The current study tested the hypothesis that ASIC3 in muscle is necessary for development of cutaneous mechanical, but not heat, hyperalgesia induced by muscle inflammation. Using mechanical and heat stimuli, we assessed behavioral responses in ASIC3-/- and ASIC3+/+ mice after induction of carrageenan muscle inflammation. ASIC3-/- mice did not develop cutaneous mechanical hyperalgesia after muscle inflammation when compared to ASIC3+/+ mice; heat hyperalgesia developed similarly between groups. We then tested if the phenotype could be rescued in ASIC3-/- mice by using a recombinant herpes virus vector to express ASIC3 in skin (where testing occurred) or muscle (where inflammation occurred). Infection of mouse DRG neurons with ASIC3-encoding virus resulted in functional expression of ASICs. Injection of ASIC3-encoding virus into muscle or skin of ASIC3-/- mice resulted in ASIC3 mRNA in DRG and protein expression in DRG and the peripheral injection site. Injection of ASIC3-encoding virus into muscle, but not skin, resulted in development of mechanical hyperalgesia similar to that observed in ASIC3+/+ mice. Thus, ASIC3 in primary afferent fibers innervating muscle is critical to development of hyperalgesia that results from muscle insult. |
Databáze: | OpenAIRE |
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