Effects of surface roughness and dimorphism on the adhesion of Candida albicans to the surface of resins: scanning electron microscope analyses of mode and number of adhesions
| Autor: | Takafumi Arimoto, Yoshizumi Yamasaki, Yukimichi Tamaki, Nobuaki Yamaguchi, Takashi Miyazaki, Ryuta Kataoka, Yuki Watanabe, Mitsuori Mayahara |
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| Rok vydání: | 2013 |
| Předmět: |
Denture Bases
Time Factors Hypha Surface Properties Scanning electron microscope Colony Count Microbial Mycology Surface type Microbiology Dental Materials Candida albicans Surface roughness Humans Polymethyl Methacrylate Composite material Mycelium biology Chemistry Biofilm Adhesiveness General Medicine Adhesion biology.organism_classification Culture Media Dental Polishing Stationary phase Biofilms Microscopy Electron Scanning |
| Zdroj: | Journal of Investigative and Clinical Dentistry. 5:307-312 |
| ISSN: | 2041-1618 |
| DOI: | 10.1111/jicd.12055 |
| Popis: | Aim Candida albicans is a common oral fungus but can cause serious conditions such as Candida stomatitis. We investigated C. albicans adhesion to the surface of denture-base resins at two growth phases. Methods Fungal suspensions of logarithmic (9 h) and stationary phase (24 h) C. albicans (JCM2085) were used. Scanning electron microscopy (SEM) confirmed that yeast and mycelial forms were predominant in 9-h and 24-h cultures, respectively. Resin strips were polished to three surface roughness levels (Ra 3.2 μm, Ra 0.48 μm and Ra 0.06 μm) and were then immersed in C. albicans suspensions for both phases. The SEM images were taken at five sites on each strip. Results Adhesion of mycelial-form C. albicans on rough surfaces (Ra = 3.2) was 2.2 times higher than on smooth surfaces (Ra = 0.06; 7030 vs 3580 adhesions/mm2, P |
| Databáze: | OpenAIRE |
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