MicroRNA-30b-5p is involved in the regulation of cardiac hypertrophy by targeting CaMKIIδ
Autor: | Hui Li, Juan He, Er-fu Chu, Shan Jiang, Fenglan Li, Mei-na Sun, Ming-Zi Chen, Xuejiao Zhao |
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Rok vydání: | 2013 |
Předmět: |
medicine.medical_specialty
Molecular Sequence Data Regulator Down-Regulation Cardiomegaly Plasma protein binding Biology General Biochemistry Genetics and Molecular Biology law.invention law Genes Reporter Internal medicine Ca2+/calmodulin-dependent protein kinase microRNA medicine Myocyte Animals Humans Myocytes Cardiac Rats Wistar Luciferases Gene 3' Untranslated Regions Cells Cultured Base Sequence General Medicine Cell biology Rats MicroRNAs Endocrinology Animals Newborn Suppressor Calcium-Calmodulin-Dependent Protein Kinase Type 2 Function (biology) Protein Binding |
Zdroj: | Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 61(3) |
ISSN: | 1708-8267 |
Popis: | Background MicroRNAs (miRNAs) participate in the regulation of cardiac hypertrophy. However, it remains largely unknown as to how miRNAs are integrated into the hypertrophic program. Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a hypertrophic signaling marker. It is not yet clear which miRNAs can regulate CaMKIIδ. Purpose In this study, we identified which miRNAs could regulate CaMKIIδ and how to regulate CaMKIIδ. Methods Through computational and expression analyses, miR-30b-5p was identified as a candidate regulator of CaMKIIδ. Quantitative expression analysis of hypertrophic models demonstrated significant down-regulation of miR-30b-5p compared with control groups. Luciferase reporter assay showed that miR-30b-5p could significantly inhibit the expression of CaMKIIδ. Moreover, through gain-of-function and loss-of-function approaches, we found miR-30b-5p could negatively regulate the expression of CaMKIIδ and miR-30b-5p was a regulator of cardiac hypertrophy. Conclusion Our study demonstrates that the expression of miR-30b-5p is down-regulated in cardiac hypertrophy, and restoration of its function inhibits the expression of CaMKIIδ, suggesting that miR-30b-5p may act as a hypertrophic suppressor. |
Databáze: | OpenAIRE |
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