Chemerin is highly expressed in hepatocytes and is induced in non-alcoholic steatohepatitis liver
| Autor: | Kristina Eisinger, Christa Buechler, Michael Beck, Josef Wanninger, Yvonne Hader, Thomas S. Weiss, Andreas Schmid, Christoph Dorn, Sabrina Krautbauer, Andrea Kopp |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male medicine.medical_specialty Clinical Biochemistry Immunoblotting Adipokine Adipose tissue Enzyme-Linked Immunosorbent Assay Real-Time Polymerase Chain Reaction Pathology and Forensic Medicine Mice Non-alcoholic Fatty Liver Disease Internal medicine medicine Hepatic Stellate Cells Chemerin Animals Humans Obesity Molecular Biology Aged Aged 80 and over biology Chemotactic Factors Chemistry Reverse Transcriptase Polymerase Chain Reaction Fatty liver Middle Aged medicine.disease Immunohistochemistry Metformin Fatty Liver Mice Inbred C57BL Disease Models Animal Endocrinology biology.protein Hepatic stellate cell Hepatocytes Intercellular Signaling Peptides and Proteins Tumor necrosis factor alpha Female Steatohepatitis Chemokines medicine.drug |
| Zdroj: | Experimental and molecular pathology. 95(2) |
| ISSN: | 1096-0945 |
| Popis: | Chemerin is a recently described adipokine whose adipose tissue and serum levels are increased in obesity. Chemerin is expressed in the liver, and here, expression of chemerin has been studied in liver cells and in non-alcoholic fatty liver disease (NAFLD) which is more often found in obesity. Chemerin is shown to be highly expressed in primary human hepatocytes (PHH) whereas hepatic stellate cells (HSC) produce only low levels of this protein. In mice fed a high fat diet hepatic chemerin mRNA but not protein is increased. Chemerin protein is comparably expressed in the liver of control animals and ob/ob mice. Rodents fed a Paigen diet or methionine-choline deficient diet (MCD) develop non-alcoholic steatohepatitis (NASH), and liver chemerin protein tends to be higher in the first and is significantly increased in the latter. Of note, MCD fed mice have similar serum chemerin levels as the respective control animals despite lower body weight. In human fatty liver and NASH liver chemerin mRNA also tends to be induced. Cytokines like TNF and adipokines with an established role in NASH do not considerably affect PHH chemerin protein. The antidiabetic drug metformin reduces cellular and soluble chemerin in PHH as has already been described in adipose tissue. In conclusion current data show that primary human hepatocytes are a major source of hepatic chemerin and increased liver chemerin in NASH may even contribute to systemic levels. |
| Databáze: | OpenAIRE |
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