Carboxyl-modified single-wall carbon nanotubes improve bone tissue formation in vitro and repair in an in vivo rat model
| Autor: | Antonio Barrientos-Durán, Nicole I. zur Nieden, Theodore I. Malinin, Juan Carlos Rodríguez-Manzaneque, Laura P. Zanello, Ellen M. Carpenter |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cellular differentiation
human allografts Nude Pharmaceutical Science Biocompatible Materials Bone tissue Regenerative Medicine Extracellular matrix bone regeneration International Journal of Nanomedicine Osteogenesis Stem Cell Research - Nonembryonic - Human Drug Discovery Nanotechnology Osteopontin Original Research Nanotubes biology Demineralized bone matrix Cell Differentiation General Medicine Anatomy Pharmacology and Pharmaceutical Sciences Allografts Cell biology medicine.anatomical_structure Stem Cell Research - Nonembryonic - Non-Human Development of treatments and therapeutic interventions Biotechnology Materials science Cell Survival Biophysics Bioengineering Cell Line Biomaterials cartilage particles Rats Nude In vivo medicine Animals Humans Nanoscience & Nanotechnology Bone regeneration Cell Shape Transplantation Osteoblasts 5.2 Cellular and gene therapies Nanotubes Carbon Cartilage Organic Chemistry Stem Cell Research Carbon Rats demineralized bone matrix Musculoskeletal biology.protein Biochemistry and Cell Biology |
| Zdroj: | International journal of nanomedicine, vol 9, iss 1 International Journal of Nanomedicine |
| Popis: | Antonio Barrientos-Durán,1,5,* Ellen M Carpenter,2 Nicole I zur Nieden,3 Theodore I Malinin,4 Juan Carlos Rodríguez-Manzaneque,5 Laura P Zanello1,* 1Department of Biochemistry, University of California Riverside, Riverside, CA, USA; 2Department of Psychiatry and Biobehavioral Sciences, UCLA School of Medicine, South Los Angeles, CA, USA; 3Department of Cell Biology and Neuroscience, Stem Cell Center, College of Natural and Agricultural Sciences, University of California Riverside, Riverside, CA, USA; 4Tissue Bank, Department of Orthopedics, University of Miami Miller School of Medicine, Miami, FL, USA; 5Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Granada, Spain *These authors contributed equally to this workAbstract: The clinical management of bone defects caused by trauma or nonunion fractures remains a challenge in orthopedic practice due to the poor integration and biocompatibility properties of the scaffold or implant material. In the current work, the osteogenic properties of carboxyl-modified single-walled carbon nanotubes (COOH–SWCNTs) were investigated in vivo and in vitro. When human preosteoblasts and murine embryonic stem cells were cultured on coverslips sprayed with COOH–SWCNTs, accelerated osteogenic differentiation was manifested by increased expression of classical bone marker genes and an increase in the secretion of osteocalcin, in addition to prior mineralization of the extracellular matrix. These results predicated COOH–SWCNTs’ use to further promote osteogenic differentiation in vivo. In contrast, both cell lines had difficulties adhering to multi-walled carbon nanotube-based scaffolds, as shown by scanning electron microscopy. While a suspension of SWCNTs caused cytotoxicity in both cell lines at levels >20 µg/mL, these levels were never achieved by release from sprayed SWCNTs, warranting the approach taken. In vivo, human allografts formed by the combination of demineralized bone matrix or cartilage particles with SWCNTs were implanted into nude rats, and ectopic bone formation was analyzed. Histological analysis of both types of implants showed high permeability and pore connectivity of the carbon nanotube-soaked implants. Numerous vascularization channels appeared in the formed tissue, additional progenitor cells were recruited, and areas of de novo ossification were found 4 weeks post-implantation. Induction of the expression of bone-related genes and the presence of secreted osteopontin protein were also confirmed by quantitative polymerase chain reaction analysis and immunofluorescence, respectively. In summary, these results are in line with prior contributions that highlight the suitability of SWCNTs as scaffolds with high bone-inducing capabilities both in vitro and in vivo, confirming them as alternatives to current bone-repair therapies. Keywords: human allografts, demineralized bone matrix, cartilage particles, bone regeneration |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|