Pratylenchus penetrans Parasitizing Potato Crops: Morphometric and Genetic Variability of Portuguese Isolates
| Autor: | Joana M. S. Cardoso, Isabel Abrantes, I. Esteves, Diogo Gil |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine molecular diversity Range (biology) cloning Zoology Plant Science 01 natural sciences Intraspecific competition Article COI 03 medical and health sciences lcsh:Botany Molecular diversity Genetic variability Ecology Evolution Behavior and Systematics Morphometrics Genetic diversity Ecology biology Phylogenetic tree morphometrics plant-parasitic nematode fungi biology.organism_classification Pratylenchus penetrans lcsh:QK1-989 030104 developmental biology potato PEST analysis ITS Plant-parasitic nematode Potato 010606 plant biology & botany Cloning |
| Zdroj: | Plants, Vol 10, Iss 603, p 603 (2021) Plants Volume 10 Issue 3 |
| Popis: | The root lesion Pratylenchus penetrans is an economically important pest affecting a wide range of plants. The morphometry of five P. penetrans isolates, parasitizing potato roots in Portugal, was compared and variability within and between isolates was observed. Of the 15 characters assessed, vulva position (V%) in females and the stylet length in both females/males showed the lowest coefficient of intra and inter-isolate variability. Moreover, DNA sequencing of the internal transcribed spacers (ITS) genomic region and cytochrome c oxidase subunit 1 (COI) gene was performed, in order to evaluate the intraspecific genetic variability of this species. ITS revealed higher isolate genetic diversity than the COI gene, with 15 and 7 different haplotypes from the 15 ITS and 14 COI sequences, respectively. Intra- and inter-isolate genetic diversity was found considering both genomic regions. The differentiation of these isolates was not related with their geographical origin. In spite of the high intraspecific variability, phylogenetic analyses revealed that both ITS region and COI gene separate P. penetrans from other related species. Our findings contribute to increasing the understanding of P. penetrans variability. |
| Databáze: | OpenAIRE |
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