Detection and analysis of membrane interactions by a biomimetic colorimetric lipid/polydiacetylene assay
| Autor: | Sofiya Kolusheva, Shoshana Rozner, William Dowhan, Raz Jelinek, Zvi Cohen, Jerry Eichler |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Polymers
Biophysics Supramolecular chemistry Biochemistry Substrate Specificity Biomimetics Escherichia coli Molecular Biology Phospholipids Ions chemistry.chemical_classification Acetylene Vesicle Ligand binding assay Polyynes Lipid metabolism Cell Biology Polymer Lipid Metabolism Polyacetylene Polymer Enzymes Membrane chemistry Metals Liposomes Colorimetry lipids (amino acids peptides and proteins) Chromatography Thin Layer Peptides Biosensor Protein Binding Polydiacetylenes |
| Zdroj: | Analytical Biochemistry. 319:96-104 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/s0003-2697(03)00278-1 |
| Popis: | We describe applications of a colorimetric assay based on supramolecular assemblies of lipid-polydiacetylene vesicles for analysis and screening of membrane interactions of lipophilic enzymes, peptides, and ions and for study of the effects of lipid composition upon membrane properties. The lipid-polymer aggregates undergo visible and quantifiable blue-to-red transitions following interfacial interactions and perturbation by varied biochemical processes. Specifically, we show that the colorimetric assay can be tuned for selective detection of enzymes reacting with different lipid species. The experiments also demonstrate that the lipid/polymer platform facilitates screening of peptide-membrane interactions in multicomponent mixtures. The colorimetric vesicles can incorporate lipid species from different cellular sources facilitating analysis of the contribution of molecular components to membrane properties and lipid interactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect