Protein differences in human pancreatic cancer cell lines with diverse metastatic potential
| Autor: | Craig M. Doremus, Harold J. Wanebo, Michael A. Schwalke, Ronald Bleday, Michael P. Vezeridis |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Mice Nude Spleen Tumor cells Tumor heterogeneity Cell Line Mice medicine Tumor Cells Cultured Animals Humans Electrophoresis Gel Two-Dimensional Neoplasm Metastasis Polyacrylamide gel electrophoresis Cellular proteins business.industry Surgery Neoplasm Proteins Molecular Weight Pancreatic Neoplasms medicine.anatomical_structure Pancreatic cancer cell Cell culture business Neoplasm Transplantation |
| Zdroj: | Archives of surgery (Chicago, Ill. : 1960). 125(4) |
| ISSN: | 0004-0010 |
| Popis: | High-resolution two-dimensional polyacrylamide gel electrophoresis was performed on the pancreatic cell lines SG, SG-R, FG, and L3.5, which when injected into the spleen of nude mice produced hepatic metastases in 0%, 20%, 64%, and 100% of the animals, respectively. A total of 981 proteins were quantitatively identified. In the highly metastatic lines, 13 proteins were present in statistically significant greater quantities, while 4 proteins were present in statistically significant greater quantities in the cell lines with a low metastatic potential. Two proteins were unique to the highly metastatic lines, while 16 proteins were unique to the lines with a low metastatic potential. These results suggest that there are considerable quantitative and qualitative differences in the cellular proteins of human pancreatic cancer cell lines with a varying metastatic potential and imply a biochemical basis to tumor heterogeneity and metastases. |
| Databáze: | OpenAIRE |
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