Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells
| Autor: | Olaf-Georg Issinger, Lars Folke Olsen, Jennifer Hochscherf, Mai Bay Stie, Barbara Guerra, Henrik Seir Thoke |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
p53
Lysis Skin Neoplasms Surface Properties Nanoparticle Human skin 02 engineering and technology 01 natural sciences Chitosan chemistry.chemical_compound Colloid and Surface Chemistry Drug Delivery Systems 0103 physical sciences Zeta potential Tumor Cells Cultured Animals Humans Physical and Theoretical Chemistry Bovine serum albumin Particle Size Melanoma Gel electrophoresis 010304 chemical physics biology Chemistry Protein Serum Albumin Bovine Surfaces and Interfaces General Medicine Hydrogen-Ion Concentration SK-mel 28 021001 nanoscience & nanotechnology Drug delivery biology.protein Biophysics Nanoparticles Cattle Tumor Suppressor Protein p53 0210 nano-technology Biotechnology |
| Zdroj: | Stie, M B, Thoke, H S, Issinger, O-G, Hochscherf, J, Guerra, B & Olsen, L F 2019, ' Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells ', Colloids and Surfaces B: Biointerfaces, vol. 174, pp. 216-223 . https://doi.org/10.1016/j.colsurfb.2018.11.005 Stie, M B, Thoke, H S, Issinger, O G, Hochscherf, J, Guerra, B & Olsen, L F 2019, ' Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells ', Colloids and Surfaces B: Biointerfaces, vol. 174, pp. 216-223 . https://doi.org/10.1016/j.colsurfb.2018.11.005 |
| DOI: | 10.1016/j.colsurfb.2018.11.005 |
| Popis: | We have successfully encapsulated two proteins, bovine serum albumin (BSA) and p53, in chitosan-tripolyphosphate (TPP) nanoparticles at various pH values from 5.5 to 6.5 and delivered the particles to human melanoma cells. The particles have diameters ranging from 180 nm to 280 nm and a zeta potential of +15 to + 40 mV. Cellular uptake of the particles by human skin melanoma cells was evaluated by: (i) fluorescence microscopy and (ii) gel electrophoresis showing that FITC-labeled BSA and p53 could be recovered in the soluble cell fraction after lysis of the cells. Our data also show that the highest cellular uptake takes place at the lowest pH as the particles have the highest positive charge under these conditions. The method we describe appears to be a general method for delivery of proteins to cells using chitosan-TPP nanoparticles as a drug delivery system, since structurally unrelated proteins such as BSA and p53 with different isoelectrical points can be encapsulated in the chitosan-TPP nanoparticles and be effectively internalized by the cells. |
| Databáze: | OpenAIRE |
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