Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment

Autor:	Kunihiko Gekko, Koichi Matsuo
Rok vydání:	2019
Předmět:	Crystallography Conformational change Circular dichroism Protein structure Materials science Membrane protein Resolution (electron density) Synchrotron radiation Nuclear magnetic resonance spectroscopy Spectroscopy
Zdroj:	Methods in Molecular Biology ISBN: 9781493995110 Methods in Molecular Biology ISBN: 9781627032742
DOI:	10.1007/978-1-4939-9512-7_12
Popis:	Circular-dichroism (CD) spectroscopy is a powerful tool for the secondary-structure analysis of proteins. The structural information obtained by CD does not have atomic-level resolution (unlike X-ray crystallography and NMR spectroscopy), but it has the great advantage of being applicable to both nonnative and native proteins in a wide range of solution conditions containing lipids and detergents. The development of synchrotron-radiation CD (SRCD) instruments has greatly expanded the utility of this method by extending the spectra to the vacuum-ultraviolet region below 190 nm and producing information that is unobtainable by conventional CD instruments. Combining SRCD data with bioinformatics provides new insight into the conformational changes of proteins in a membrane environment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::992f0408ce88f1f746e44e59f103e863 https://doi.org/10.1007/978-1-4939-9512-7_12 Zobrazit plný text záznamu