Zinc Oxide Tetrapods Based Biohybrid Interface for Voltammetric Sensing of Helicobacter pylori
| Autor: | D.K. Avasthi, Shaivya Gupta, Rainer Adelung, Utkarsh Jain, Yogendra Kumar Mishra, Nidhi Chauhan |
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| Rok vydání: | 2018 |
| Předmět: |
Working electrode
Materials science Ion beam chemistry.chemical_element Biosensing Techniques 02 engineering and technology Zinc 010402 general chemistry Electrochemistry 01 natural sciences Limit of Detection Humans General Materials Science Irradiation Electrodes Detection limit Helicobacter pylori Electrochemical Techniques 021001 nanoscience & nanotechnology Amperometry 0104 chemical sciences chemistry Electrode Zinc Oxide 0210 nano-technology Nuclear chemistry |
| Zdroj: | ACS Applied Materials & Interfaces. 10:30631-30639 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.8b08901 |
| Popis: | Helicobacter pylori is a Gram-negative, spiral shaped, microaerophilic bacteria that colonizes human gastric mucosa and causes various gastric diseases. In this work, the utilization of ion irradiated zinc oxide tetrapods (ZnO-T) based biohybrid interface accentuates the development of an electrochemical immunosensor for the fast and sensitive detection of H. pylori. After coating of (ZnO-T) over the surface of screen printed electrode (SP-AuE) through electrodeposition, the ZnO-T/SP-AuE was irradiated with N2+ ion of energy 100 keV. The ion irradiation significantly enhances the conductivity of ZnO-T coated SP-AuE. The revamped SP-AuE is further used for establishing an immunosensor interface based upon immobilization of the CagA antigen on ZnO-T electrodeposited over the surface of SP-AuE. The sensing interface demonstrated good linearity (0.2 ng/mL to 50 ng/mL) and limit of detection (0.2 ng/mL). The ion beam irradiated ZnO-T based immunosensor showed significantly high conductivity and enhanced the analytical properties of the working electrode in terms of the sensitivity, detection limit, and response time. A study on the comparison of irradiated and pristine electrode is performed for amperometric sensing of H. pylori. In addition, the significance of work conducted on ion irradiated ZnO-T based interfaces provides a basis of further development of electrochemical immunosensors. |
| Databáze: | OpenAIRE |
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