Novel oligosaccharides isolated from Fusarium oxysporum L. rapidly induce PAL activity in Rubus cells
| ISSN: | 0001-527X |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9928ca4646d7f4f44ae92a2abf9eccf3 https://pubmed.ncbi.nlm.nih.gov/15448725 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....9928ca4646d7f4f44ae92a2abf9eccf3 |
| Autor: | Yvette Lienart, Mihai Nita-Lazar, Isabelle Braccini, Alain Heyraud, Claude Gey |
| Přispěvatelé: | Centre de Recherches sur les Macromolécules Végétales (CERMAV), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF) |
| Rok vydání: | 2004 |
| Předmět: |
Magnetic Resonance Spectroscopy
Disaccharide Phenylalanine ammonia-lyase Biology Spectrometry Mass Fast Atom Bombardment Disaccharides General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Fusarium Fusarium oxysporum Inositol Phenylalanine ammonia-lyase activity Rosaceae ComputingMilieux_MISCELLANEOUS Mycelium Phenylalanine Ammonia-Lyase Plant Diseases Molecular Structure food and beverages Fast atom bombardment biology.organism_classification Elicitor Biochemistry chemistry Enzyme Induction Signal Transduction |
| Zdroj: | Acta Biochim. Pol. Acta Biochim. Pol., 2004, pp.625-634 |
| ISSN: | 0001-527X |
| Popis: | Activation of the phenolic pathway is known to be part of a defense response against cell wall-derived elicitors from pathogens. Many examples of a defense response by increasing the synthesis of phenolic compound against the elicitor were demonstrated in the past, but the elicitor structure has so far been poorly characterized. Our results indicate that a disaccharide fraction containing the following structure: alpha-D-mannopyranosyl (1-->2)alpha/beta-D-glucopyranosyl and alpha-D-mannopyranosyl (1-->x) inositol, isolated from Fusarium oxysporum L., promotes rapid and transient phenylalanine ammonia lyase activity in Rubus fructicosus cells at nanomolar concentration. The disaccharides were isolated by size-exclusion chromatography directly from extracts obtained by alkaline treatment of F. oxysporum mycelium. Their structure was determined by 500-MHz-1H-NMR spectroscopy combined with methylation analysis and fast atom bombardment mass spectrometry. |
| Databáze: | OpenAIRE |
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