Similarity of copper and technetium binding sites in human IgG
| Autor: | Paul O. Zamora, Michael J. Marek, B.A. Rhodes, L.D. Schulte, J.A. Mercer-Smith |
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| Rok vydání: | 1992 |
| Předmět: |
Binding Sites
Chromatography biology Chemistry Technetium chemistry.chemical_element Negative control Gamma globulin General Medicine Binding Competitive Copper Molecular biology Immunoglobulin G Immunoradiotherapy In vivo biology.protein Humans gamma-Globulins Binding site Chromatography High Pressure Liquid |
| Zdroj: | International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology. 19:797-802 |
| ISSN: | 0883-2897 |
| DOI: | 10.1016/0883-2897(92)90142-l |
| Popis: | Kits for direct labeling of IgG with 99mTc were used without modification for the preparation of [67Cu]IgG. The IgG was pre-treated to generate thiolate groups which would bind 67Cu. The direct labeling of reduced IgG with 67Cu was highly efficient, resulting in approx. 95% 67Cu binding. Non-reduced IgG (negative control) had labeling efficiencies of less than 10%. IgG pre-exposed to Cu(II) had reduced amounts of 99mTc bound to it. The results demonstrate a direct relationship between copper- and 99mTc-binding sites in IgG. |
| Databáze: | OpenAIRE |
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