Paradigm of Monoterpene (β-phellandrene) Hydrocarbons Production via Photosynthesis in Cyanobacteria
| Autor: | Anastasios Melis, José G. García-Cerdán, Hsu-Ching Chen, Fiona K. Bentley |
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| Rok vydání: | 2013 |
| Předmět: |
Cyanobacteria
Lavandula angustifolia biology Renewable Energy Sustainability and the Environment Microorganism Synechocystis Biomass beta-phellandrene biology.organism_classification Photosynthesis Industrial Biotechnology Product inhibition Biofuels Botany Monoterpenes Bioenergy Heterologous expression Agronomy and Crop Science Energy (miscellaneous) Biotechnology |
| Zdroj: | BioEnergy Research, vol 6, iss 3 |
| Popis: | A direct "photosynthesis-to-fuels" approach envisions application of a single organism, absorbing sunlight, photosynthesizing, and converting the primary products of photosynthesis into ready-made fuel. The work reported here applied this concept for the photosynthetic generation of monoterpene (β-phellandrene) hydrocarbons in the unicellular cyanobacteria Synechocystis sp. PCC 6803. Heterologous expression of a codon-optimized Lavandula angustifolia β-phellandrene synthase (β-PHLS) gene in Synechocystis enabled photosynthetic generation of β-phellandrene in these microorganisms. β-phellandrene accumulation occurred constitutively and in tandem with biomass accumulation, generated from sunlight, CO2, and H2O. Results showed that β-phellandrene diffused through the plasma membrane and cell wall of the cyanobacteria and accumulated on the surface of the liquid culture. Spontaneous β-phellandrene separation from the biomass and its removal from the liquid phase alleviated product inhibition of cellular metabolism and enabled a continuous production process. The work showed that oxygenic photosynthesis can be directed to generate monoterpene hydrocarbons, while consuming CO2, without a prior requirement for the harvesting, dewatering, and processing of the respective biomass. © 2013 Springer Science+Business Media New York. |
| Databáze: | OpenAIRE |
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