Neochloris oleoabundans is worth its salt : Transcriptomic analysis under salt and nitrogen stress

Autor: B.M. Carreres, Gerrit Eggink, Dirk E. Martens, Vitor A. P. Martins dos Santos, Peter J. Schaap, René H. Wijffels, Lenny de Jaeger, Jan Springer
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Sucrose
Bio Process Engineering
Magnetic Resonance Spectroscopy
Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 [VDP]
lcsh:Medicine
Biomass
Marine and Aquatic Sciences
Fresh Water
Sodium Chloride
Disaccharides
Biochemistry
Starches
chemistry.chemical_compound
Chlorophyta
Microalgae
Systems and Synthetic Biology
Food science
Amino Acids
lcsh:Science
chemistry.chemical_classification
Systeem en Synthetische Biologie
Multidisciplinary
biology
Organic Compounds
Fatty Acids
Starch
Genomics
Lipids
Glutathione
Chemistry
Osmolyte
Physical Sciences
Osmoregulation
Transcriptome Analysis
Research Article
Freshwater Environments
Proline
Nitrogen
Carbohydrates
Salt (chemistry)
Biosynthesis
03 medical and health sciences
Stress
Physiological
Genetics
Life Science
VLAG
Gene Expression Profiling
lcsh:R
Ecology and Environmental Sciences
Organic Chemistry
Chemical Compounds
Computational Biology
Aquatic Environments
Biology and Life Sciences
Proteins
Molecular Sequence Annotation
Cyclic Amino Acids
Neochloris oleoabundans
biology.organism_classification
Genome Analysis
Biosynthetic Pathways
Oxidative Stress
030104 developmental biology
BBP Bioconversion
Bioprocess engineering
chemistry
Earth Sciences
lcsh:Q
Salts
Transcriptome
Peptides
Zdroj: PLoS ONE 13 (2018) 4
PLoS ONE
PLoS ONE, Vol 13, Iss 4, p e0194834 (2018)
PLoS ONE, 13(4)
ISSN: 1932-6203
Popis: Neochloris oleoabundans is an oleaginous microalgal species that can be cultivated in fresh water as well as salt water. Using salt water gives the opportunity to reduce production costs and the fresh water footprint for large scale cultivation. Production of triacylglycerols (TAG) usually includes a biomass growth phase in nitrogen-replete conditions followed by a TAG accumulation phase under nitrogen-deplete conditions. This is the first report that provides insight in the saline resistance mechanism of a fresh water oleaginous microalgae. To better understand the osmoregulatory mechanism of N. oleoabundans during growth and TAG accumulating conditions, the transcriptome was sequenced under four different conditions: fresh water nitrogen-replete and -deplete conditions, and salt water (525 mM dissolved salts, 448mM extra NaCl) nitrogen-replete and -deplete conditions. In this study, several pathways are identified to be responsible for salt water adaptation of N. oleoabundans under both nitrogen-replete and -deplete conditions. Proline and the ascorbate-glutathione cycle seem to be of importance for successful osmoregulation in N. oleoabundans. Genes involved in Proline biosynthesis were found to be upregulated in salt water. This was supported by Nuclear magnetic resonance (NMR) spectroscopy, which indicated an increase in proline content in the salt water nitrogen-replete condition. Additionally, the lipid accumulation pathway was studied to gain insight in the gene regulation in the first 24 hours after nitrogen was depleted. Oil accumulation is increased under nitrogen-deplete conditions in a comparable way in both fresh and salt water. The mechanism behind the biosynthesis of compatible osmolytes can be used to improve N. oleoabundans and other industrially relevant microalgal strains to create a more robust and sustainable production platform for microalgae derived products in the future.
Databáze: OpenAIRE
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