Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries

Autor: Talita de Mello Santos, Diego de Oliveira, Lucas Silva Côrtes, Marilia Martins Cavariani, Henrique Spaulonci Silveira, Luiz Antonio Lupi, Letícia Barbosa Gaiotte, Raquel Fantin Domeniconi, Luiz Gustavo de Almeida Chuffa, Luis A. Justulin
Přispěvatelé: Universidade Estadual Paulista (Unesp)
Rok vydání: 2021
Předmět:
Male
0301 basic medicine
Aging
medicine.medical_treatment
p38 Mitogen-Activated Protein Kinases
PI3K
030226 pharmacology & pharmacy
Phosphatidylinositol 3-Kinases
0302 clinical medicine
Ovarian Follicle
Follicular phase
General Pharmacology
Toxicology and Pharmaceutics
Gonadal Steroid Hormones
media_common
Estrous cycle
TOR Serine-Threonine Kinases
DOHaD
General Medicine
Maternal restriction
medicine.anatomical_structure
mTOR
Animal Nutritional Physiological Phenomena
Female
Folliculogenesis
Corpus luteum
Signal Transduction
medicine.medical_specialty
Offspring
media_common.quotation_subject
Estrous Cycle
Ovary
Biology
Low-protein diet
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Internal medicine
Diet
Protein-Restricted
medicine
Animals
Rats
Wistar
Ovulation
AKT
Ovarian tissue
MAPK
030104 developmental biology
Endocrinology
Proto-Oncogene Proteins c-akt
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 0024-3205
Popis: Made available in DSpace on 2021-06-25T11:06:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Aims: Because an adequate protein supply is detrimental for the maintenance of folliculogenesis and ovulation, we evaluated the impact of maternal low protein diet on nutritional parameters, estrous cycle, ovarian histomorphometry, and on the expression of metabolic and survival signaling molecules in different follicular stages. Main methods: Twenty Wistar pregnant rats were divided into two groups: the normoprotein (NP) group, composed of animals that received 17% protein, and a low-protein (LP) group, composed of animals that received 6% protein during gestation and lactation period. After weaning, female rats were fed with standard diet until the 120-days-old. Key findings: LP animals showed reduced body mass index, total body weight, energy intake, feed efficiency, and visceral fat. The ovarian tissue presented vascular congestion and fat accumulation in the medulla, followed by a significant reduction in the amount of primordial and primary follicles. In addition, the number of atretic follicles was higher in LP than in NP animals. Maternal undernutrition also resulted in increased levels of estradiol (E2) and progesterone (P4) while testosterone (T) was unchanged in the offspring. Although discrete changes in p38MAPK and in PI3K-AKT-mTOR immunostaining were observed in the ovarian follicles and corpus luteum in LP, no differences were found at their protein levels. Significance: Maternal protein restriction alters estrous cycle and histomorphometry of the offspring's ovary without changing the levels of intracellular regulatory molecules in adulthood. These morphofunctional changes may alter reproductive performance in female offspring, highlighting maternal dietary conditions as an important factor for offspring reproductive health. Faculty of Medicine of Botucatu São Paulo State University-UNESP Department of Structural and Functional Biology Institute of Biosciences (IBB) São Paulo State University-UNESP Faculty of Medicine of Botucatu São Paulo State University-UNESP Department of Structural and Functional Biology Institute of Biosciences (IBB) São Paulo State University-UNESP FAPESP: 2018/02224-7
Databáze: OpenAIRE
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