Morphometric analysis of cultured hepatocytes exposed to benoxaprofen
| Autor: | Elsie M. B. Sorensen |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Pathology
medicine.medical_specialty Cell type Dose-Response Relationship Drug L-Lactate Dehydrogenase Anti-Inflammatory Agents Non-Steroidal Benoxaprofen Rats Inbred Strains General Medicine Biology Toxicology Rats Functional integrity Liver Morphometric analysis medicine Animals Propionates Cytotoxicity Cells Cultured medicine.drug |
| Zdroj: | Toxicology Letters. 34:277-286 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/0378-4274(86)90220-1 |
| Popis: | This study was undertaken to quantitate morphological changes in primary cultures of liver cells exposed to benoxaprofen and to compare morphological assessments with standard assessments of functional integrity. Rat hepatocytes were exposed for 12 h to 0, 100, 500, or 1000 μM concentrations of benoxaprofen and subjected to double-blind morphometric analysis. As benoxaprofen concentration was increased from 0 to 1000 μM, the relative percentage of morphologically normal cells was reduced, whereas the percentages of damaged cell types increased. These results paralleled those obtained from functional assessments. Therefore, the use of morphometric techniques provided a good assessment of benoxaprofen-induced cytotoxicity and demonstrated the value of quantitative estimates in the evaluation of cellular injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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