Hydrogen sulfide formation in experimental model of acute pancreatitis

Autor: Maria Wróbel, Klaudia Maksymiuk, Patrycja Bronowicka-Adamska, Marta Gawryś-Kopczyńska, Tomasz Hutsch
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
cystathionine-beta-synthase
acute pancreatitis
Proteolysis
sulfane sulfur
Cystine
hydrogen sulfide
Cystathionine beta-Synthase
Gene Expression
Sulfurtransferase
Rhodanese
Rats
Inbred WKY
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
Cystathionine
Bile Ducts
Extrahepatic
medicine
Animals
Cysteine
Hydrogen Sulfide
Ligation
Pancreas
Transaminases
Cysteine metabolism
3-mercaptopyruvate sulfurtransferase
0303 health sciences
biology
medicine.diagnostic_test
030302 biochemistry & molecular biology
Cystathionine gamma-Lyase
Pancreatic Ducts
Glutathione
Cystathionine beta synthase
Thiosulfate Sulfurtransferase
Mitochondria
Rats
Disease Models
Animal
Pancreatitis
chemistry
Biochemistry
Sulfurtransferases
biology.protein
gammacystathionase
Thiosulfate sulfurtransferase
rhodanese
Popis: Acute pancreatitis (AP) is a disease defined as acute or chronic inflammatory process of the pancreas characterized by premature activation of digestive enzymes within the pancreatic acinar cells and causing pancreatic auto-digestion. In mammalian tissues, H2S is synthesized endogenously from L-cysteine in regulated enzymatic pathways catalyzed by pyridoxal phosphate-dependent enzymes: cystathionine beta - synthase (CBS), gamma - cystathionase (CTH) and cysteine aminotransferase (CAT) coupled with 3-mercaptopyruvate sulfurtransferase (MPST). In the mitochondria, hydrogen sulfide is oxidized to sulfite, which is then converted to thiosulfate (sulfane sulfur-containing compound) by thiosulfate sulfurtransferase (rhodanese; TST). The activity and the expression of CBS, CTH, MPST, and TST have been determined in vivo in pancreas of control rats, rats with acute pancreatitis and sham group. Levels of low-molecular sulfur compounds such as reduced and oxidized glutathione, cysteine, cystine and cystathionine were also determined. The study showed the significant role of MPST in H2S metabolism in pancreas. Stress caused by the surgery (sham group) and AP cause a decrease in H2S production associated with a decrease of MPST activity and expression. Markedly higher level of cysteine in the AP pancreas may be caused by a reduced rate of cysteine consumption in reaction catalyzed by MPST but it can also be a sign of the processes of proteolysis occurring in the changed tissue.
Databáze: OpenAIRE
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