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Additional file 1: Figure S1. Initial and finalTEM images of AuMu biosynthesis in various concentrations of PGM (10mg, 20mg, 30mg, 40mg and 50 mg in solution volume of 7 mL for pH 3 & pH 9 and 6mL for pH6) and at pH3, 6 and 9. Figure S2. TEM images of reaction kinetic phases of the AuMuN Pbiosynthesis under pH3 environment.A) The synthsis starts (phaseone) by the formation of spherical particles under k 1reaction rate.B) The fusion of spherical particles into complex triangular particles under k2 reaction rate in phase two. Figure S3. Kinetic analysis from the UV-V is data obtained from synthesis reaction at different PGM concentrations at different reaction times. The rate constant k was obtained from the slope of graph of logarithm of maximum AuNP plasmonic absorption peak at the final stage of synthesis reaction minus maximum AuMu plasmonic absorption peak at the beginning of the reaction infront of the reaction time [5, 45, 46]. The indication of time when the reaction began is the transition of the color from the Au ions + PGM solution: from pale yellow to pink (pH3), bluish (pH6) or red (pH9). Figure S4. Photothermalproperties of the AuMu complexes biosynthesized at different PGM concentration and pH.A) 1.7mg/mlPGM, B) 3.3mg/ml PGM, C) 5mg/mlPGM, D) 6.7mg/mlPGM, E) 8.3mg/ml PGM. The data show the maximum temperature reached under NIR laser irradiation vs. time of the reaction (errorof±1ºCand±1 min). Figure S5. MRSA growth in TS-broth over time in the presence of 1mg/mL of pure mucin. The MRSA inoculations were prepared at different starting concentrations (105,106, and 107) with control representing a sterile solution. Figure S6. MRSA growth vs. 808 nm laser irradiation for 0, 1, 3, and 5 min in the presence of 6x10-12 MauNPs (70 nm, biotinterminated). |
