Cell Stiffening in Response to External Stress is Correlated to Actin Recruitment
| Autor: | Olivier Cardoso, Delphine Icard-Arcizet, Alain Richert, Sylvie Hénon |
|---|---|
| Přispěvatelé: | Henon, Sylvie, Matière et Systèmes Complexes (MSC (UMR_7057)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Materials science
Optical Tweezers [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Biophysics Mechanotransduction Cellular Cell Line Myoblasts 03 medical and health sciences Mice Micromanipulation 0302 clinical medicine Fluorescence microscope medicine Cell Adhesion Myocyte Animals Humans Elasticity (economics) [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] Cell adhesion Actin ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] [PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] Stiffness [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] Epithelial Cells Actins Elasticity Stiffening Cell biology Optical tweezers Cell Biophysics Stress Mechanical medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Biophysical Journal Biophysical Journal, Biophysical Society, 2008, 94 (7), pp.2906-2913 Biophysical Journal, Biophysical Society, 2008, 94, pp.2906-2913 |
| ISSN: | 0006-3495 1542-0086 |
| Popis: | We designed a micromanipulation device that allows the local application of a constant force on living cells, and the measurement of their stiffness. The force is applied through an Arg-Gly-Asp-coated bead adhering on the cell and trapped in optical tweezers controlled by a feedback loop. Epifluorescence observations of green fluorescent protein-actin in the cells are made during force application. We observe a stiffening of cells submitted to a constant force within a few minutes, coupled to actin recruitment both at the bead-cell contact and up to several micrometers from the stress application zone. Moreover, kinetics of stiffening and actin recruitment exhibit a strong correlation. This work presents the first quantification of the dynamics of cell mechanical reinforcement under stress, which is a novel insight into the elucidation of the more general phenomenon of cell adaptation to stress. |
| Databáze: | OpenAIRE |
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