The glycolytic flux in Escherichia coli is controlled by the demand for ATP
| Autor: | Dan Nilsson, Brian J. Koebmann, Jacky L. Snoep, Peter Ruhdal Jensen, Hans V. Westerhoff |
|---|---|
| Přispěvatelé: | Molecular Cell Physiology |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Anabolism
ATP synthase biology Catabolism Physiology and Metabolism Metabolism Microbiology Proton-Translocating ATPases chemistry.chemical_compound Adenosine Triphosphate chemistry Biochemistry ATP hydrolysis Escherichia coli biology.protein Glycolysis Promoter Regions Genetic Molecular Biology Adenosine triphosphate Flux (metabolism) |
| Zdroj: | Koebmann, B J, Westerhoff, H V, Snoep, J L, Nilsson, D & Jensen, P R 2002, ' The glycolytic flux in Escherichia coli is controlled by the demand for ATP. ', Journal of Bacteriology, vol. 184, pp. 3909-3916 . https://doi.org/10.1128/JB.184.14.3909-3916.2002 Journal of Bacteriology, 184, 3909-3916. American Society for Microbiology Technical University of Denmark Orbit |
| ISSN: | 0021-9193 |
| DOI: | 10.1128/JB.184.14.3909-3916.2002 |
| Popis: | The nature of the control of glycolytic flux is one of the central, as-yet-uncharacterized issues in cellular metabolism. We developed a molecular genetic tool that specifically induces ATP hydrolysis in living cells without interfering with other aspects of metabolism. Genes encoding the F 1 part of the membrane-bound (F 1 F 0 ) H + -ATP synthase were expressed in steadily growing Escherichia coli cells, which lowered the intracellular [ATP]/[ADP] ratio. This resulted in a strong stimulation of the specific glycolytic flux concomitant with a smaller decrease in the growth rate of the cells. By optimizing additional ATP hydrolysis, we increased the flux through glycolysis to 1.7 times that of the wild-type flux. The results demonstrate why attempts in the past to increase the glycolytic flux through overexpression of glycolytic enzymes have been unsuccessful: the majority of flux control (>75%) resides not inside but outside the pathway, i.e., with the enzymes that hydrolyze ATP. These data further allowed us to answer the question of whether catabolic or anabolic reactions control the growth of E. coli . We show that the majority of the control of growth rate resides in the anabolic reactions, i.e., the cells are mostly “carbon” limited. Ways to increase the efficiency and productivity of industrial fermentation processes are discussed. |
| Databáze: | OpenAIRE |
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