Effects of Phospholipase C Inhibitors on Ca2+Channel Stimulation and Ca2+Release from Intracellular Stores Evoked by α1A- and α2A-Adrenoceptors in Rat Portal Vein Myocytes

Autor: Arnaudeau, S., Boittin, F.X., Macrez-Leprêtre, Nathalie, Morel, Jean-Luc, Mironneau, Jean
Přispěvatelé: Laboratoire de Physiologie Cellulaire et Pharmacologie Moléculaire, URA CNRS 1489, Université de Bordeaux II, France, Institut des Maladies Neurodégénératives [Bordeaux] (IMN), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie Cellulaire et Pharmacologie Moléculaire, INSERM CJF 88-13, Université de Bordeaux II, France.
Jazyk: angličtina
Rok vydání: 1996
Předmět:
Patch-Clamp Techniques
[SDV]Life Sciences [q-bio]
Stimulation
030204 cardiovascular system & hematology
Pharmacology
Biochemistry
Muscle
Smooth
Vascular
Membrane Potentials
chemistry.chemical_compound
Norepinephrine
0302 clinical medicine
Enzyme Inhibitors
Estrenes
Inositol phosphate
ComputingMilieux_MISCELLANEOUS
chemistry.chemical_classification
0303 health sciences
Portal Vein
Norbornanes
Pyrrolidinones
3. Good health
Intracellular
Bridged-Ring Compounds
medicine.medical_specialty
Adrenergic receptor
Biophysics
Alpha (ethology)
Biology
In Vitro Techniques
03 medical and health sciences
Receptors
Adrenergic
alpha-2
Thiocarbamates
Internal medicine
Receptors
Adrenergic
alpha-1
medicine
Animals
Patch clamp
Phosphatidylinositol
Rats
Wistar
Molecular Biology
030304 developmental biology
Phospholipase C
Dose-Response Relationship
Drug
Thiones
Cell Biology
Rats
Endocrinology
chemistry
Type C Phospholipases
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Calcium
Calcium Channels
Zdroj: Biochemical and Biophysical Research Communications
Biochemical and Biophysical Research Communications, Elsevier, 1996, 218 (1), pp.30-34. ⟨10.1006/bbrc.1996.0006⟩
ISSN: 0006-291X
1090-2104
Popis: The ability of phospholipase C inhibitors to inhibit Ca2+ channel stimulation and Ca2+ release from intracellular stores evoked by norepinephrine in single rat portal vein myocytes was investigated in the aim of identifying the type of phospholipase C involved in the transduction pathways activated by alpha 1A- and alpha 2A-adrenoceptors. U73122 (an inhibitor of phosphatidylinositol-phospholipase C) inhibited in a concentration-dependent manner the release of Ca2+ from the intracellular stores induced by activation of alpha 1A-adrenoceptors and related to inositol phosphate production whereas U73343 was ineffective. Both compounds had no effect on the release of Ca2+ induced by caffeine. However, U73122 and U73343 inhibited the L-type Ca2+ channel. D609 (an inhibitor of phosphatidylcholine-phospholipase C) had no direct inhibitory effects on the L-type Ca2+ channel but it inhibited concentration dependently the alpha 2A-adrenoceptor-induced stimulation of Ca2+ channels, which had been shown to be independent of phosphatidylinositol hydrolysis. Therefore, these results suggest that alpha 2A-adrenoceptors activate a phosphatidylcholine-phospholipase C in vascular myocytes. However, D609 had other sites of action as it blocked norepinephrine- and caffeine-induced Ca2+ release from the intracellular stores.
Databáze: OpenAIRE
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