BNP gene expression is specifically modulated by stretch and ET-1 in a new model of isolated rat atria
| Autor: | Leonardo A. Piazza, Adolfo J. de Bold, Benoit G. Bruneau |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Transcriptional Activation medicine.medical_specialty Transcription Genetic Physiology Nerve Tissue Proteins Biology Immediate-Early Proteins Proto-Oncogene Proteins c-myc Rats Sprague-Dawley Physiology (medical) Internal medicine Natriuretic Peptide Brain Gene expression medicine Animals Secretion Heart Atria RNA Messenger cardiovascular diseases Rat atria Early Growth Response Protein 1 Endothelin-1 Myocardium Heart Brain natriuretic peptide Myocardial Contraction Endothelin 1 In vitro Rats DNA-Binding Proteins Kinetics Endocrinology Circulatory system cardiovascular system Cardiology and Cardiovascular Medicine Endothelin receptor Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists Transcription Factors |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 273:H2678-H2686 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1997.273.6.h2678 |
| Popis: | We have assessed the effects of stretch or endothelin-1 (ET-1) on atrial natriuretic factor (ANF) and brain natriuretic peptide (BNP) secretion and gene expression using a new model of isolated right atria from the rat. This model allows for comparatively long-term in vitro study of adult tissue while retaining the anatomic conformation of the atrium. Stretch and ET-1 resulted in a transient stimulation of ANF and BNP secretion, with an initially larger proportional increase in ANF release. Stretch and ET-1 induced a marked increase in BNP gene expression after 1.5 and 4 h, respectively; the increase in BNP mRNA levels was maintained throughout the 8-h experimental period. Stretch and ET-1 also stimulated c- myc and Egr-1 mRNA levels, two markers of mechanical and receptor-mediated transcriptional activation. The selective response of the BNP gene to stretch and ET-1 and the distinct responses of ANF and BNP secretion indicate that the atrial cardiocytes have the capability to individually regulate the synthesis of its endocrine products. This suggests that each hormone plays a specific role in the response of the heart to hemodynamic or neuroendocrine imbalances. |
| Databáze: | OpenAIRE |
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