Characterization of a Helicobacter pylori neutrophil-activating protein
| Autor: | D.N. Granger, David Y. Graham, H C Lampert, P R Kvietys, Dolores G. Evans, T Takemura, Doyle J. Evans, H Nakano |
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| Rok vydání: | 1995 |
| Předmět: |
Free Radicals
Protein subunit Molecular Sequence Data Immunology CD18 Inflammation Microbiology Bacterial Adhesion Neutrophil Activation Gene product Bacterial Proteins medicine Humans Helicobacter Base Sequence Helicobacter pylori biology CD11 Antigens biology.organism_classification In vitro Molecular Weight Oxygen Infectious Diseases Integrin alpha M Genes Bacterial CD18 Antigens biology.protein Parasitology medicine.symptom Research Article |
| Zdroj: | Infection and Immunity. 63:2213-2220 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Helicobacter pylori-associated gastritis is mainly an inflammatory cell response. In earlier work we showed that activation of human neutrophils by a cell-free water extract of H. pylori is characterized by increased expression of neutrophil CD11b/CD18 and increased adhesiveness to endothelial cells. The work reported here indicates that the neutrophil-activating factor is a 150,000-molecular-weight protein (150K protein). Neutrophil proadhesive activity copurified with this protein, which is a polymer of identical 15K subunits. Specific antibody, prepared against the purified 15K subunit, neutralized the proadhesive activity of the pure protein and of water extracts obtained from different strains of H. pylori. The gene (napA) for this protein (termed HP-NAP, for H. pylori neutrophil-activating protein) was detected, by PCR amplification, in all of the H. pylori isolates tested; however, there was considerable strain variation in the level of expression of HP-NAP activity in vitro. HP-NAP could play an important role in the gastric inflammatory response to H. pylori infection. |
| Databáze: | OpenAIRE |
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