Evaluation of In Vivo Osteogenic Potential of Bone Morphogenetic Protein 2-Overexpressing Human Periodontal Ligament Stem Cells Combined with Biphasic Calcium Phosphate Block Scaffolds in a Critical-Size Bone Defect Model
| Autor: | Su Jin Kim, Jeong-Ho Yun, Choong-Man Jun, TacGhee Yi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Scaffold Periodontal ligament stem cells Periodontal Ligament Biomedical Engineering Bone Morphogenetic Protein 2 Bioengineering Gene delivery Biochemistry Bone morphogenetic protein 2 Bone and Bones Adenoviridae Biomaterials Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine In vivo Osteogenesis Transduction Genetic Animals Humans Bone regeneration Tissue Scaffolds Chemistry Stem Cells Cell Differentiation 030206 dentistry Immunohistochemistry In vitro Cell biology Transplantation Disease Models Animal 030104 developmental biology Female Hydroxyapatites Biomedical engineering Stem Cell Transplantation |
| Zdroj: | Tissue engineering. Part A. 22(5-6) |
| ISSN: | 1937-335X |
| Popis: | Human periodontal ligament stem cells (hPDLSCs) are considered potential cellular carriers for gene delivery in the field of tissue regeneration. This study tested the osseoregenerative potential of hPDLSCs transduced with replication-deficient recombinant adenovirus (rAd) containing the gene encoding bone morphogenetic protein-2 (BMP2; hPDLSCs/rAd-BMP2) in both in vivo and in vitro osteogenic environments. After the optimal condition for rAd-mediated transduction was determined, hPDLSCs were transduced to express BMP2. In vivo bone formation was evaluated in a critical-size rat calvarial bone defect model that more closely mimics the harsher in vivo milieu for bone regeneration than subcutaneous transplantation model. As support materials for bone regeneration, block-type biphasic calcium phosphate (BCP) scaffolds were combined with hPDLSCs and/or BMP2 and transplanted into critical-size bone defects in rats. Experimental groups were as follows: BCP scaffold control (group 1 [Gr1]), scaffold containing recombinant human BMP2 (rhBMP2; group 2 [Gr2]), scaffold loaded with normal hPDLSCs (group 3 [Gr3]), scaffold combined with both normal hPDLSCs and rhBMP2 (group 4 [Gr4]), and scaffold loaded with hPDLSCs transduced with rAd-BMP2 (hPDLSCs/rAd-BMP2; group 5 [Gr5]). Our data showed that new bone formation was highest in Gr2. Less mineralization was observed in Gr3, Gr4, and Gr5 in which hPDLSCs were transplanted. In vitro transwell assay demonstrated that hPDLSCs exert an inhibitory activity on BMP2-induced osteogenic differentiation. Our findings suggest that the in vivo bone regenerative potential of BMP2-overexpressing hPDLSCs could be compromised in a critical-size rat calvarial bone defect model. Thus, further investigations are required to elucidate the underlying mechanisms and to develop efficient techniques for improved tissue regeneration. |
| Databáze: | OpenAIRE |
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