Fabrication of cell container arrays with overlaid surface topographies
| Autor: | Stefan Giselbrecht, H.V. Unadkat, Bernke J. Papenburg, Roman Truckenmüller, Jan de Boer, Matthias Wessling, Volker Saile, Dimitrios Stamatialis, Max N.W. Groenendijk, Nicolas C. Rivron, Albert van den Berg, Vinod Subramaniam, Clemens van Blitterswijk, Maryana Escalante-Marun |
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| Přispěvatelé: | Executive board Vrije Universiteit, Nanobiophysics, Biomaterials Science and Technology, Faculty of Science and Technology, Membrane Science & Technology |
| Rok vydání: | 2012 |
| Předmět: |
Fabrication
Materials science Polymers Surface Properties Polyesters Microthermoforming Biomedical Engineering Polymer films Nanotechnology Thermoforming IR-79976 Container (type theory) Research Support METIS-283267 Article Cell Line Mice Immune Regulation [NCMLS 2] Honeycomb Journal Article Polylactic acid (PLA) Animals Lactic Acid Nanoimprint Non-U.S. Gov't Nanoscopic scale Molecular Biology Microscale chemistry chemistry.chemical_classification EWI-21696 Tissue Engineering Research Support Non-U.S. Gov't Polymer High-Throughput Screening Assays chemistry Microwell arrays |
| Zdroj: | Biomedical Microdevices, 14(1), 95-107. Kluwer Academic Publishers Biomedical microdevices, 14(1), 95-107. Kluwer Academic Publishers Biomedical Microdevices Truckenmüller, R, Giselbrecht, S, Escalante-Marun, M, Groenendijk, M, Papenburg, B, Rivron, N, Unadkat, H, Saile, V, Subramaniam, V, van den Berg, A, van Blitterswijk, C, Wessling, M, Boer, J D & Stamatialis, D 2012, ' Fabrication of cell container arrays with overlaid surface topographies ', Biomedical Microdevices, vol. 14, no. 1, pp. 95-107 . https://doi.org/10.1007/s10544-011-9588-5 Biomedical Microdevices, 14, 1, pp. 95-107 Biomedical Microdevices, 14, 95-107 |
| ISSN: | 1387-2176 |
| Popis: | This paper presents cell culture substrates in the form of microcontainer arrays with overlaid surface topographies, and a technology for their fabrication. The new fabrication technology is based on microscale thermoforming of thin polymer films whose surfaces are topographically prepatterned on a micro- or nanoscale. For microthermoforming, we apply a new process on the basis of temporary back moulding of polymer films and use the novel concept of a perforated-sheet-like mould. Thermal micro- or nanoimprinting is applied for prepatterning. The novel cell container arrays are fabricated from polylactic acid (PLA) films. The thin-walled microcontainer structures have the shape of a spherical calotte merging into a hexagonal shape at their upper circumferential edges. In the arrays, the cell containers are arranged densely packed in honeycomb fashion. The inner surfaces of the highly curved container walls are provided with various topographical micro- and nanopatterns. For a first validation of the microcontainer arrays as in vitro cell culture substrates, C2C12 mouse premyoblasts are cultured in containers with microgrooved surfaces and shown to align along the grooves in the three-dimensional film substrates. In future stem-cell-biological and tissue engineering applications, microcontainers fabricated using the proposed technology may act as geometrically defined artificial microenvironments or niches. Electronic supplementary material The online version of this article (doi:10.1007/s10544-011-9588-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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