Determination of asymmetric dimethylarginine, an endogenous nitric oxide synthase inhibitor, in umbilical blood
| Autor: | Fumikazu Kotsuji, Shuko Tokuriki, Norihito Ohta, Hirokazu Tsukahara, Hironori Nagasaka, Kyouichi Sekine, Hisako Kawakami, Naoko Ohta, Mitsufumi Mayumi, Koji Nishijima |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male medicine.medical_specialty Umbilical Veins Adolescent Nitric Oxide Synthase Type III Endocrinology Diabetes and Metabolism Vasodilation Arginine Preeclampsia Nitric oxide chemistry.chemical_compound Endocrinology Pregnancy Internal medicine medicine Humans Enzyme Inhibitors Child Nitrites Fetus Nitrates business.industry Infant Newborn Middle Aged Delivery mode medicine.disease Fetal Blood chemistry Child Preschool Circulatory system Linear Models Female business Asymmetric dimethylarginine Infant Premature |
| Zdroj: | Metabolism: clinical and experimental. 57(2) |
| ISSN: | 0026-0495 |
| Popis: | Endothelial cells produce nitric oxide (NO), a potent vasodilator. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NO synthase. Little is known about the potential physiological roles of ADMA in a perinatal setting. This study measures concentrations of ADMA in umbilical blood using enzyme-linked immunosorbent assay and those of NO as nitrite/nitrate (NOx(-)) using the Griess assay. Their relationship to the degree of prematurity and maternal clinical condition is examined. Results show that ADMA concentrations in umbilical blood from control newborns were about twice as high as those of lactating women, healthy children, and healthy adults. Umbilical blood NOx(-) concentrations from control newborns were about half of those of lactating women, healthy children, and healthy adults. Consequently, the levels of ADMA relative to NOx(-) were about 4-fold higher in umbilical blood from control newborns than in blood from lactating women, healthy children, and healthy adults. Furthermore, the umbilical blood ADMA concentrations and the ratios of ADMA to NOx(-) in newborns were higher according to their birth prematurity and lower birth weight. The umbilical ADMA concentrations were independent of the delivery mode and maternal preeclampsia. We infer that the high ADMA levels play physiological roles in maintaining vascular tone and blood redistribution to vital organs during birth, thereby favoring the circulatory transition from fetal to neonatal life. |
| Databáze: | OpenAIRE |
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