Targeted lysis of HIV-infected cells by natural killer cells armed and triggered by a recombinant immunoglobulin fusion protein: implications for immunotherapy
| Autor: | Sergei Radaev, Prateeti Khazanie, Anthony S. Fauci, Domenico Mavilio, Peter Schuck, Shyam Kottilil, Claudia Cicala, Donald Van Ryk, James Arthos, Marybeth Daucher, Ronald L. Rabin, Peter D. Sun, Eva Chung, Neil Gupta, Nina Censoplano, Catherine C. Cruz, Tavis D. Steenbeke, Margery A. Chaikin |
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| Rok vydání: | 2005 |
| Předmět: |
Natural killer cell
HIV Infections Interleukin 21 Immune system NK-92 Antigens CD Cell Line Tumor Virology medicine Humans Calcium Signaling Antibody dependent cellular cytotoxicity Lymphokine-activated killer cell biology Receptors IgG Antibody-Dependent Cell Cytotoxicity HIV Flow Cytometry Fusion protein Killer Cells Natural medicine.anatomical_structure Recombinant antibody Immunoglobulin G biology.protein Interleukin 12 Immunotherapy Antibody CD16 |
| Zdroj: | Virology. 332:491-497 |
| ISSN: | 0042-6822 |
| Popis: | Natural killer (NK) cells play an important role in both innate and adaptive antiviral immune responses. The adaptive response typically requires that virus-specific antibodies decorate infected cells which then direct NK cell lysis through a CD16 mediated process termed antibody-dependent cellular cytotoxicity (ADCC). In this report, we employ a highly polymerized chimeric IgG1/IgA immunoglobulin (Ig) fusion protein that, by virtue of its capacity to extensively crosslink CD16, activates NK cells while directing the lysis of infected target cells. We employ HIV as a model system, and demonstrate that freshly isolated NK cells preloaded with an HIV gp120-specific chimeric IgG1/IgA fusion protein efficiently lyse HIV-infected target cells at picomolar concentrations. NK cells pre-armed in this manner retain the capacity to kill targets over an extended period of time. This strategy may have application to other disease states including various viral infections and cancers. |
| Databáze: | OpenAIRE |
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